PrimeWay Gel Extraction/ PCR Purification II Kit is an improved version of our original kit, designed for faster and easier DNA purification. With a ready-to-use silica spin column, DNA can be purified in just 16 minutes with high recovery.
The kit removes unwanted components such as primers, dNTPs, enzymes, salts, and short PCR fragments (< 65 bp), providing DNA that is ready for downstream applications like sequencing, PCR, cloning, and DNA labeling.
FEATURES
- Fast - 10 min for PCR Purification; 20 min for Gel Extraction
- High recovery - Up to 95% for gel extraction & PCR clean-up
- Efficient - Removal of reaction contaminants including enzymes, proteins, primers dimers, dNTPs, and salts
- Convenience - No calculation required for BD buffer amount if gel slice ≤300mg
APPLICATION DATA
Improvement on PrimeWay Gel Extraction/ PCR Purification II Kit
In PCR clean-up, PrimeWay II shows around 10% higher recovery at the smallest (65 bp) and largest (13 kb) fragment sizes.
In gel extraction, the PrimeWay II with 70°C heated elution demonstrates consistently higher DNA recovery compared to the previous PrimeWay I kit across nearly the entire fragment size range. The improvement is most notable for mid-sized fragments (1.5 to 3 kb), while even smaller fragments (~200 bp) show an additional 10 to 29% increase in yield.
PrimeWay II consistently delivers matching or higher DNA recovery than the PrimeWay I kit across both TAE (Tris-Acetate-EDTA) and TBE (Tris-Borate-EDTA) gels. Recovery is further enhanced when using 70°C heated elution with PrimeWay II.
PCR product with fragment size of 723 bp was directly purified using PCR clean up protocol in triplicate and quantified by spectrophotometer. At smallest elution volume of 12µL, the DNA concentration ~20ng/µL, with approximately 50% of total DNA recovery maintained.
Removal of Contaminants
PCR products (200 bp, 723 bp, 1 kb, 1.5 kb) and restriction-digested plasmid fragments (2.5 kb, 3 kb, 3.7 kb, 8 kb, 13 kb) were efficiently recovered from TAE agarose gels using the PrimeWay Gel Extraction/ PCR Purification II Kit (KIT-9051). Analysis on a 1% TAE gel with Floro+Red Nucleic Acid Stain (BIO-5171-600µl) confirms sharp, clear bands across all sizes. The results are comparable with the leading brand.
Yield Recovery
DNA yield recovery performance from PrimeWay II enhanced Gel Extraction Protocol compared to competitor brand across both TAE (Tris-Acetate-EDTA) and TBE (Tris-Borate-EDTA) gels. PrimeWay II pre-heated elution buffer at 70°C, consistently matches or outperforms major brands across TAE & TBE systems.
Experience superior DNA recovery and purity with the enhanced Gel Extraction Protocol. Utilizing a pre-heated elution buffer at 70°C, PrimeWay II matches the performance of the standard Brand Q and significantly outperforms room-temperature elution across medium and large DNA fragments. At room temperature, it offers recovery akin to Brand P, with the added benefit of enhanced recovery at 70 °C for all fragment sizes.
Removal of Contaminants
Purified PCR products (65 bp, 200 bp, 723 bp, 1 kb, 1.5 kb) and restriction-digested plasmid fragments (2.5 kb, 3 kb, 3.7 kb, 8 kb, 13 kb) were efficiently recovered from TAE agarose gels using the PrimeWay Gel Extraction/ PCR Purification II Kit (KIT-9051). Analysis on a 1% TAE gel with Floro+Red Nucleic Acid Stain (BIO-5171-600µl) confirms sharp, clear bands across all sizes. The results are comparable with the leading brand.
Removal of Primer Dimer
PCR amplicons of 723 bp and 1.5 kb were purified using the PrimeWay Gel Extraction/ PCR Purification II Kit (KIT-9051). For quality assessment, 1 µL of unpurified PCR product (lane 1) and purified PCR product (lane 2) were analyzed on a 1% TAE agarose gel stained with Floro+Red Nucleic Acid Stain (BIO-5171-600µL). The gel confirmed that the purified PCR products were of the expected sizes and free from detectable contaminants, making them suitable for downstream applications.
Up to 100% Yield
PrimeWay II delivers DNA yield and purity on par with Brand Q and Brand P, ensuring maximum recovery and reliability for your downstream applications.
Yield & Purity
10 µL of the 100 bp DNA Ladder (1st BASE, BIO-5130-50ug) was purified using various PCR Purification kits. DNA was eluted in 50 µL of Elution Buffer, and 1 µL of the purified product was analyzed on a 1.7% TAE gel stained with Floro+Red Nucleic Acid Stain (BIO-5171-600ul). The results highlight the recovery performance of PrimeWay II is higher as compared to the Brand P and Brand Q.
10 µL of the 1 kb DNA Ladder (1st BASE, BIO-5130-50ug) was purified using various PCR Purification kits. DNA was eluted in 50 µL of Elution Buffer, and 1 µL of the purified product was analyzed on a 1.7% TAE gel stained with Floro+Red Nucleic Acid Stain (BIO-5171-600ul). The results highlight the recovery performance of PrimeWay II is higher as compared to the Brand P and Brand Q.
| Product Code | KIT-9051 |
| Product | PrimeWay Gel Extraction – PCR Purification Kit II |
| DNA Size | 65 bp to 15 kb |
| Binding Capacity | 20 µg |
| Size | 10/50/250 preps |
| Sample Type | Unpurified PCR product |
| Yield/Recovery | Up to 100% |
| Elution | 30 - 50 µL |
| Duration | PCR Purification: 6 min; Gel Extraction: 16 min |
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