If you don’t bother to get very clean and long read of your cloned DNA, you may consider our Direct Colony Sequencing (DCS). No plasmid extraction or rolling circle amplification is required.
If your plasmid DNA is from single colony, visible at the size of 1 mm x 6 mm on agar, we can directly sequence the inserted DNA with your vector sequencing primer. Although this compromises the read length (up to 700 bases), but the fast turnaround time worth of this consideration. High copy plasmid DNA has higher successful rate with our DCS, merely because the provided colony size has higher amount of inserted DNA for direct sequencing.
Each single colony + 1 vector sequencing primer = 1 Direct Colony Sequencing reaction
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The following is the list of complimentary Universal Primers offered by our DNA Sequencing facility.
| Universal Primers | Sequence (5' - 3') |
| 1492R | 5' TACGGYTACCTTGTTACGACTT 3' |
| 27F | 5' AGAGTTTGATCMTGGCTCAG 3' |
| 35S-A | 5' AAGGGTCTTGCGAAGGATAG 3' |
| 35S-B | 5' AGTGGAAAAGGAAGGTGGCT 3' |
| 518F | 5' CCAGCAGCCGCGGTAATACG 3' |
| 800R | 5' TACCAGGGTATCTAATCC 3' |
| ACYCDuetUP1 | 5' GGATCTCGACGCTCTCCCT 3' |
| AD Reverse | 5' AGATGGTGCACGATGCACAG 3' |
| a-Factor | 5' TACTATTGCCAGCATTGCTGC 3' |
| AOX1 Forward | 5' GACTGGTTCCAATTGACAAGC 3' |
| AOX1 Reverse | 5' GCAAATGGCATTCTGACATCC 3' |
| BGH-R | 5' TAGAAGGCACAGTCGAGG 3' |
| Bluescript KS | 5' TCGAGGTCGACGGTATC 3' |
| Bluescript SK | 5' CGCTCTAGAACTAGTGGATC 3' |
| CMV-F | 5' CGCAAATGGGCGGTAGGCGTG 3' |
| CMV-profor | 5' ATGGGCGGTAGGCGTG 3' |
| CYC1 Reverse | 5' GCGTGAATGTAAGCGTGAC 3' |
| DsRed1-C | 5' AGCTGGACATCACCTCCCACAACG 3' |
| DsRed1-N | 5' GTACTGGAACTGGGGGGACAG 3' |
| DuetDown1 | 5' GATTATGCGGCCGTGTACAA 3' |
| EBV-RP | 5' GTGGTTTGTCCAAACTCATC 3' |
| EGFP-C | 5' CATGGTCCTGCTGGAGTTCGTG 3' |
| EGFP-CF | 5' AGCACCCAGTCCGCCCTGAGC 3' |
| EGFP-CR | 5' CGTCCATGCCGAGAGTG 3' |
| EGFP-N | 5' CGTCGCCGTCCAGCTCGACCAG 3' |
| EGFP-NR | 5' CGTCGCCGTCCAGCTC 3' |
| GAL1 Forward | 5' AATATACCTCTATACTTTAACGTC 3' |
| Gal4AD | 5' TACCACTACAATGGATG 3' |
| GLprimer1 | 5' TGTATCTTATGGTACTGTAACTG 3' |
| GLprimer2 | 5' CTTTATGTTTTTGGCGTCTTCCA 3' |
| HCO2198 | 5' TAAACTTCAGGGTGACCAAAAAATCA 3' |
| hU6-F Primer | 5’-GAGGGCCTATTTCCCATGATT-3’ |
| ITS1 | 5' TCCGTAGGTGAACCTGCGG 3' |
| ITS2 | 5' GCTGCGTTCTTCATCGATGC 3' |
| ITS3 | 5' GCATCGATGAAGAACGCAGC 3' |
| ITS4 | 5' TCCTCCGCTTATTGATATGC 3' |
| ITS5 | 5' GGAAGTAAAAGTCGTAACAAGG 3' |
| KAN2-FP | 5' ACCTACAACAAAGCTCTCATCAACC 3' |
| KAN2-RP | 5' GCAATGTAACATCAGAGATTTTGAG 3' |
| LCO1490 | 5' GGTCAACAAATCATAAAGATATTGG 3' |
| LpJET1.2F | 5' CTGCTTTAACACTTGTGCCTGA 3' |
| LpJET1.2R | 5' TTCCTGATGAGGTGGTTAGCAT 3' |
| M13F (-20) | 5' GTAAAACGACGGCCAGT 3' |
| M13F (-29) | 5' CACGACGTTGTAAAACGAC 3' |
| M13-FP | 5' TGTAAAACGACGGCCAGT 3' |
| M13F-pUC(-40) | 5' GTTTTCCCAGTCACGAC 3' |
| M13R (-20) | 5' GCGGATAACAATTTCACACAGG 3' |
| M13R (-24) | 5' GGAAACAGCTATGACCATG 3' |
| M13R-pUC (-26) | 5' CAGGAAACAGCTATGAC 3' |
| MT Forward | 5' CATCTCAGTGCAACTAAA 3' |
| pBacPAC-RP | 5' GTCTGTAAATCAACAACGC 3' |
| pBAD-F | 5' ATGCCATAGCATTTTTATCCA 3' |
| pBAD-FP | 5' ATGCCATAGCATTTTTATCC 3' |
| pBAD-R | 5' GATTTAATCTGTATCAGG 3' |
| pBRrevBam | 5' GGTGATGTCGGCGATATAGG 3' |
| pDONOR-FP | 5' TAACGCTAGCATGGATCTC 3' |
| pEGFP_N | 5' CCGTCCAGCTCGACCAG 3' |
| pEGFP-FP | 5' TTTAGTGAACCGTCAGATC 3' |
| pEGFP-RP | 5' AACAGCTCCTCGCCCTTG 3' |
| pESP-RP | 5' TCCAAAAGAAGTCGAGTGG 3' |
| pET-24a | 5' GGGTTATGCTAGTTATTGCTCAG 3' |
| pET-RP | 5' CTAGTTATTGCTCAGCGG 3' |
| pFastBac Forward | 5' GGATTATTCATACCGTCCCA 3' |
| pFastBac Reverse | 5' CAAATGTGGTATGGCTGATT 3' |
| pGEX3 | 5' GGAGCTGCATGTGTCAGAGG 3' |
| pGEX5 | 5' GGCAAGCCACGTTTGGTG 3' |
| pJET1.2F | 5' CGACTCACTATAGGGAGAGCGGC 3' |
| pJET1.2R | 5' AAGAACATCGATTTTCCATGGCAG 3' |
| pMalE | 5' TCAGACTGTCGATGAAGC 3' |
| pQE-F | 5' CCCGAAAAGTGCCACCTG 3' |
| pQE-R | 5' GTTCTGAGGTCATTACTGG 3' |
| pREP-fwd | 5' GCTCGATACAATAAACGCC 3' |
| pRH Forward | 5' CTGTCTCTATACTCCCCTATAG 3' |
| pRH Reverse | 5' CAAAATTCAATAGTTACTATCGC 3' |
| pTrcHis Forward | 5' GAGGTATATATTAATGTATCG 3' |
| QE Promoter | 5' CCGAAAAGTGCCACCTG 3' |
| RVprimer3 | 5' CTAGCAAAATAGGCTGTCCC 3' |
| RVprimer4 | 5' GACGATAGTCATGCCCCGCG 3' |
| SP6 | 5' ATTTAGGTGACACTATAG 3' |
| STag 18mer Primer | 5' GAACGCCAGCACATGGAC 3' |
| SV40-pArev | 5' CCTCTACAAATGTGGTATGG 3' |
| SV40-Promoter | 5' GCCCCTAACTCCGCCCATCC 3' |
| T3 | 5' ATTAACCCTCACTAAAG 3' |
| T7 | 5' AATACGACTCACTATAG 3' |
| T7 EEV | 5' ATGTCGTAATAACCCCGCCCCG 3' |
| T7promoter | 5' TAATACGACTCACTATAGGG 3' |
| T7terminator | 5' GCTAGTTATTGCTCAGCGG 3' |
| U-19mer Primer | 5' GTTTTCCCAGTCACGACGT 3' |
| U6 Primer | 5’-GGGCAGGAAGAGGGCCTAT-3’ |
| SP6Long | 5'-ATTTAGGTGACACTATAGAATAC-3' |
| T7Long | 5'-GTAATACGACTCACTATAGGGC-3' |
| LpJet1_2F | 5' CTGCTTTAACACTTGTGCCTGA 3' |
| LpJet1_2R | 5' TTCCTGATGAGGTGGTTAGCAT 3' |
| 785F | 5' GGATTAGATACCCTGGTA 3' |
| 907R | 5' CCGTCAATTCMTTTRAGTTT 3' |
Guides on Preparations and Requirements
Sample RequirementsList of Services & DetailsGuidelines to Sample Preparation and Quantification