AmpliPLUS Q SYBR qPCR Mix is a 2X master mix containing the dye SYBR Green I, designed for real-time qPCR reactions. It includes a hot-start DNA polymerase in a unique qPCR buffer system, which reduces the amplification of non-specific products and enhances amplification efficiency by offering high specificity and sensitivity. The mix is suitable for templates rich in AT and GC content.

Most qPCR instruments are compatible with the included ROX Reference Dye, eliminating the need for ROX calibration across different systems. Additionally, AmpliPLUS Q SYBR qPCR Mix does not produce bubbles during vortexing. The product remains stable after 20 freeze-thaw cycles and can be stored at 37°C for an extended period of time.

• Multi-platform Compatibility: Formulated with a specific ROX, AmpliPLUS Q SYBR qPCR Mix is compatible with most qPCR instruments, eliminating the need for calibration across different devices.
• High Sensitivity: AmpliPLUS Q SYBR qPCR Mix delivers a strong linear relationship across a wide quantitative range, allowing for the detection of templates with concentrations as low as pg-level.
• Superior Specificity: The unique combination of the qPCR buffer system and hot start enzyme effectively suppresses the production of non-specific products, enhancing amplification specificity.
• Exceptional Stability: AmpliPLUS Q SYBR qPCR Mix maintains stable performance even after 20 repeated freeze-thaw cycles and can be stored at 37°C for 7 days

Specifications

Superior Overall Performance

1 ng of human genome DNA was used as a template to amplify 3 different genes.

AmpliPLUS Q SYBR qPCR Mix has better overall performance than competing products in different amplification systems, with excellent sensitivity, high fluorescence value and good specificity.

Broad Quantitative Range

The target gene was detected by diluting it in 6 gradients at a 10-fold ratio using plasmid as template.

AmpliPLUS Q SYBR qPCR Mix enables a good linear relationship across a wide quantitative range.

Universal Compatibility Across Multiple Platforms

The target gene was detected by diluting it with 2 gradients and 10-fold ratio on different instruments using plasmid as template.

AmpliPLUS Q SYBR qPCR Mix can be used on different instruments with an excellent result. It is suitable for most qPCR instruments and no ROX calibration is required.

Efficient Amplification in a Compact System

 
3 target gene was detected by diluting the template with 2 gradients and 10-fold ratio using human genome as template in a 10 μL system.

AmpliPLUS Q SYBR qPCR Mix can be successfully amplified in a 10 μL system for different target genes, suggesting its applicability to small amplification systems.

AmpliPLUS Q Probe Master Mix is a 2X master mix designed for real-time qPCR reactions, compatible with probes like TaqMan and Molecular Beacon. It contains a dual-antibody modified hot-start DNA polymerase in a unique qPCR buffer system, which minimises non-specific amplification and enhances single and multiple amplification efficiency with high specificity and sensitivity, detecting template concentrations as low as 30 pg.

• Enhanced sensitivity and amplification efficiency: The dual-antibody modification significantly boosts the detection rate of low-concentration templates, enabling detection with as little as 30 pg of initial DNA.
• Extensive linear detection range: Accurate measurements are possible across up to 6 logarithmic dynamic ranges, ensuring precise results.
• Compatibility with AT and GC content: Suitable for templates rich in AT and GC base pairs
• Impressive storage stability: Maintains stable performance even after storage at 37°C for 14 days and undergoing 30 repeated freeze-thaw cycles.

Specifications

Superior Amplification & Sensitivity

Bisulfite-treated DNA served as the template for two gradients of 10-fold dilution. AmpliPLUS Q Probe qPCR Mix was utilised for comparison with similar products from Company A, Company B, and Company C.

Human 293T cell DNA was used as the template with a DNA input of 30 pg, and AmpliPLUS Q Probe qPCR Mix was applied to detect multiple target genes.

AmpliPLUS Q Probe qPCR Mix exhibited superior amplification sensitivity, achieving a 100% detection rate for low templates.

Optimised for AT and GC Rich Template

Human 293T cell DNA served as the template for two gradients of 10-fold dilution. AmpliPLUS Q Probe qPCR Mix was employed for the specific detection of models with high AT (65%) and GC (73.9%) content.

AmpliPLUS Q Probe qPCR Mix demonstrates excellent amplification performance for AT- and GC-rich templates and boasts high compatibility.

Extensive Linear Detection Range

Human 293T cell DNA was utilised as the template for six gradients of 10-fold dilution, and AmpliPLUS Q Probe qPCR Mix was employed to detect the target gene.

AmpliPLUS Q Probe qPCR Mix provides precise measurements across up to 6 logarithmic dynamic ranges, ensuring the accuracy of the analysis.

Excellent Storage Stability

After undergoing 30 freeze-thaw cycles, AmpliPLUS Q Probe qPCR Mix was used to detect target genes following dilution of bisulfite-treated DNA in two 10-fold gradients.

AmpliPLUS Q Probe qPCR Mix was incubated at 4°C, 25°C, and 37°C for 14 days each. Fluorescence quantitative detections were then performed using DNA treated with bisulfite as the template to compare these storage conditions with those at -20°C.

AmpliPLUS Q Probe qPCR Mix demonstrates stable storage performance at temperatures of 4°C, 25°C, and 37°C for 14 days, as well as after undergoing 30 repeated freeze-thaw cycles.

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