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ExactMark 100bp DNA Ladder, Ready-To-Use

Product Series: BIO-5130
Used for sizing and approximate quantification of wide range double-stranded DNA on agarose gel. The ladder is composed of eleven individual DNA fragments (in base pairs): 1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200 and 100. It contains two reference bands of 1500 and 500bp for easy orientation. Orange G and xylene cyanol FF are used as tracking dyes.


Storage Buffer

10mM Tris-HCl (pH 8.0)
1mM EDTA


Protocol for Loading

    • Load 5µl of the solution into a 5mm agarose gel lane.
    • It is recommended to load 1μl (0.1μg) of DNA ladder per 1mm of gel lane.


Recommendations

    • Do not heat solution prior to loading.
    • Adjust sample concentration to be approximately equal to the amount of DNA in the nearest band of the ladder for quantification purpose.
    • Visualize DNA by staining with DNA stain (C/No: BIO-5170-1ml).

ExactMark 1kb DNA Ladder, Ready-To-Use

Product Series: BIO-5140

Used for sizing and approximate quantification of wide range double-stranded DNA on agarose gel. The ladder is composed of thirteen individual DNA fragments (in base pairs): 10000, 8000, 6000, 5000, 4000, 3000, 2500, 2000, 1500, 1000, 750, 500, and 250. It contains three reference bands of, 3000 and 1000bp for easy orientation. Bromophenol blue is used as tracking dye.

Storage Buffer

10mM Tris-HCl (pH 8.0)
1mM EDTA

Protocol for Loading

    • Load 5µl of the solution into a 5mm agarose gel lane.
    • It is recommended to load 1μl (0.1μg) of DNA ladder per 1mm of gel lane.

Recommendations

    • Do not heat solution prior to loading.
    • Adjust sample concentration to be approximately equal to the amount of DNA in the nearest band of the ladder for quantification purpose.

Interested to try it out?

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Product Description No of Applications Product No. Product Manual Safety Data Sheet
ExactMark 100bp DNA Ladder, Ready-To-Use, 50ug ~100 applications BIO-5130-50ug
ExactMark 100bp DNA Ladder, Ready-To-Use, 100ug ~200 applications BIO-5130-100ug
ExactMark 1kb DNA Ladder, Ready-To-Use, 50ug ~100 applications BIO-5140-50ug
ExactMark 1kb DNA Ladder, Ready-To-Use, 100ug ~200 applications BIO-5140-100ug
ExactMark 1kb DNA Ladder, Ready-To-Use, 250ug ~500 applications BIO-5140-250ug
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Product Description Grade Packaging Product No. Product Specification Sheet Safety Data Sheet
Agarose Biotechnology Grade 100g BIO-1000-100g
500g BIO-1000-500g
1kg BIO-1000-1kg
Water DEPC treated Biotechnology Grade 200ml BUF-1170-200ml
1L BUF-1170-1L
Water Biotechnology Grade 100ml BUF-1180-100ml
500ml BUF-1180-500ml
1L BUF-1180-1L
1X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Ultra Pure Grade 1L BUF-3000-1X1L
10X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Ultra Pure Grade 1L BUF-3000-10X1L
4L BUF-3000-10X4L
50X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Ultra Pure Grade 1L BUF-3000-50X1L
4L BUF-3000-50X4L
10X Tris-Acetate-EDTA (TAE) Buffer pH 7.8 Ultra Pure Grade 1L BUF-3001-10X1L
10X Tris-Acetate-EDTA (TAE) Buffer Premixed Powder Ultra Pure Grade 1L BUF-3002-10X1L
1X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Biotechnology Grade 1L BUF-3003-1X1L
10X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Biotechnology Grade 100ml BUF-3003-10X100ml
1L BUF-3003-10X1L
10X Tris-Acetate-EDTA (TAE) Buffer Premixed Powder (Sachet) Ultra Pure Grade 1L BUF-3009-10X1L
1X Tris-Borate-EDTA (TBE) Buffer pH 8.3 Ultra Pure Grade 1L BUF-3010-1X1L
10X Tris-Borate-EDTA (TBE) Buffer pH 8.3 Ultra Pure Grade 1L BUF-3010-10X1L
4L BUF-3010-10X4L
10X Tris-Borate-EDTA (TBE) Buffer Premixed Powder Ultra Pure Grade 1L BUF-3011-10X1L
10X Tris-Borate-EDTA (TBE) Buffer pH 8.3 Biotechnology Grade 100ml BUF-3013-10X100ml
500ml BUF-3013-10X500ml
1L BUF-3013-10X1L
10X Tris-Borate-EDTA (TBE) Buffer Premixed Powder (Sachet) Ultra Pure Grade 1L BUF-3019-10X1L
1X Tris-EDTA (TE) Buffer pH8.0 Ultra Pure Grade 1L BUF-3020-1X1L
10X Tris-EDTA (TE) Buffer pH 8.0 Ultra Pure Grade 1L BUF-3020-10X1L
4L BUF-3020-10X4L
1X Tris-EDTA (TE) Buffer with reduced EDTA pH 8.0 Ultra Pure Grade 1L BUF-3021-1X1L
10X Tris-EDTA (TE) Buffer with reduced EDTA pH 8.0 Ultra Pure Grade 1L BUF-3021-10X1L
1X Tris-EDTA (TE) Buffer pH 8.0 Biotechnology Grade 100ml BUF-3024-1X100ml
500ml BUF-3024-1X500ml
0.1M EDTA Solution pH 7.4 Ultra Pure Grade 500ml BUF-1051-500ml-pH7.4
1L BUF-1051-1L-pH7.4
0.1M EDTA Solution pH 8.0 Ultra Pure Grade 500ml BUF-1051-500ml-pH8.0
1L BUF-1051-1L-pH8.0
0.5M EDTA Solution pH 8.0 Ultra Pure Grade 500ml BUF-1052-500ml-pH8.0
1L BUF-1052-1L-pH8.0
0.5M EDTA Solution pH 8.0 Biotechnology Grade 100ml BUF-1053-100ml-pH8.0
1L BUF-1053-1L-pH8.0
5.0M Sodium Chloride Solution Biotechnology Grade 500ml BUF-1112-500ml
1L BUF-1112-1L
1X SSC Buffer Biotechnology Grade 1L BUF-3050-1X1L
20X SSC Buffer Biotechnology Grade 1L BUF-3050-20X1L
20X SSC Buffer Ultra Pure Grade 1L BUF-3051-20X1L
1X SSPE Buffer Biotechnology Grade 1L BUF-3060-1X1L
20X SSPE Buffer Biotechnology Grade 1L BUF-3060-20X1L
Sterile Deionized Water Biotechnology Grade 1L BUF-4100-1L