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ExactMark 100bp DNA Ladder, Ready-To-Use

Product Series: BIO-5130
Used for sizing and approximate quantification of wide range double-stranded DNA on agarose gel. The ladder is composed of eleven individual DNA fragments (in base pairs): 1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200 and 100. It contains two reference bands of 1500 and 500bp for easy orientation. Orange G and xylene cyanol FF are used as tracking dyes.


Storage Buffer

10mM Tris-HCl (pH 8.0)
1mM EDTA


Protocol for Loading

    • Load 5µl of the solution into a 5mm agarose gel lane.
    • It is recommended to load 1μl (0.1μg) of DNA ladder per 1mm of gel lane.


Recommendations

    • Do not heat solution prior to loading.
    • Adjust sample concentration to be approximately equal to the amount of DNA in the nearest band of the ladder for quantification purpose.
    • Visualize DNA by staining with DNA stain (C/No: BIO-5170-1ml).

ExactMark 1kb DNA Ladder, Ready-To-Use

Product Series: BIO-5140

Non-carcinogenic alternative of Ethidium Bromide, DNA Stain can be used as a safer alternative compared to the traditional ethidium bromide stain used for DNA and RNA detection in agarose gel. Compared to ethidium bromide, it has low toxicity and mutagenicity. DNA Stain has two secondary fluorescence excitation peaks (~270 nm; ~295 nm) and one strong excitation peak centered around 490 nm. The fluorescence emission is similar to Ethidium Bromide when bound to DNA which is at about 530 nm.

Usage Protocols

Precasting method:

    • Prepare 100ml of 0.8-3.0% agarose gel solution.
    • Heat till solution is completely clear.
    • Add 4-6µl of DNA stain and mix gently with gel.
    • Cool gel to 50-60°C and cast gel into gel tray.
    • Load samples into solidified gel and perform electrophoresis.
    • Detect bands under UV illuminator.

Post-staining method:

    • For agarose gel less than 0.5cm thickness, 10 – 25µl of the stain should be used per 100ml of buffer
    • Staining time is optimized between 5 – 60 minutes; amount of stain varies with gel thickness and concentration of agarose.
Description No of Applications Product No. Protocol MSDS
ExactMark 100bp DNA Ladder, Ready-To-Use, 50ug ~100 applications BIO-5130-50ug
ExactMark 100bp DNA Ladder, Ready-To-Use, 100ug ~200 applications BIO-5130-100ug
ExactMark 1kb DNA Ladder, Ready-To-Use, 50ug ~100 applications BIO-5140-50ug
ExactMark 1kb DNA Ladder, Ready-To-Use, 100ug ~200 applications BIO-5140-100ug
ExactMark 1kb DNA Ladder, Ready-To-Use, 250ug ~500 applications BIO-5140-250ug
Online Ordering

1st BASE Online Ordering, 1oo (strongly recommended)

1st BASE Order Online simple as 1-2-3.
Try it now! It is amazingly easy to use and hassle-free!
To find out more about how to use 1oo, please click here.


For ordering or enquiries, please contact us as follow:

Singapore
Email: custcare@axilscientific.com
Tel: +65 6775 7318

Malaysia / International
Email: custcare@apicalscientific.com
Tel: +603 8943 3252

Product Description Grade Packaging Product No Product Specification Sheet Safety Data Sheet
Agarose Biotechnology Grade 100g BIO-1000-100g
500g BIO-1000-500g
1kg BIO-1000-1kg
Water DEPC treated Biotechnology Grade 200ml BUF-1170-200ml
1L BUF-1170-1L
Water Biotechnology Grade 100ml BUF-1180-100ml
500ml BUF-1180-500ml
1L BUF-1180-1L
1X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Ultra Pure Grade 1L BUF-3000-1X1L
10X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Ultra Pure Grade 1L BUF-3000-10X1L
4L BUF-3000-10X4L
50X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Ultra Pure Grade 1L BUF-3000-50X1L
4L BUF-3000-50X4L
10X Tris-Acetate-EDTA (TAE) Buffer pH 7.8 Ultra Pure Grade 1L BUF-3001-10X1L
10X Tris-Acetate-EDTA (TAE) Buffer Premixed Powder Ultra Pure Grade 1L BUF-3002-10X1L
1X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Biotechnology Grade 1L BUF-3003-1X1L
10X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Biotechnology Grade 100ml BUF-3003-10X100ml
1L BUF-3003-10X1L
10X Tris-Acetate-EDTA (TAE) Buffer Premixed Powder (Sachet) Ultra Pure Grade 1L BUF-3009-10X1L
1X Tris-Borate-EDTA (TBE) Buffer pH 8.3 Ultra Pure Grade 1L BUF-3010-1X1L
10X Tris-Borate-EDTA (TBE) Buffer pH 8.3 Ultra Pure Grade 1L BUF-3010-10X1L
4L BUF-3010-10X4L
10X Tris-Borate-EDTA (TBE) Buffer Premixed Powder Ultra Pure Grade 1L BUF-3011-10X1L
10X Tris-Borate-EDTA (TBE) Buffer pH 8.3 Biotechnology Grade 100ml BUF-3013-10X100ml
500ml BUF-3013-10X500ml
1L BUF-3013-10X1L
10X Tris-Borate-EDTA (TBE) Buffer Premixed Powder (Sachet) Ultra Pure Grade 1L BUF-3019-10X1L
1X Tris-EDTA (TE) Buffer pH8.0 Ultra Pure Grade 1L BUF-3020-1X1L
10X Tris-EDTA (TE) Buffer pH 8.0 Ultra Pure Grade 1L BUF-3020-10X1L
4L BUF-3020-10X4L
1X Tris-EDTA (TE) Buffer with reduced EDTA pH 8.0 Ultra Pure Grade 1L BUF-3021-1X1L
10X Tris-EDTA (TE) Buffer with reduced EDTA pH 8.0 Ultra Pure Grade 1L BUF-3021-10X1L
1X Tris-EDTA (TE) Buffer pH 8.0 Biotechnology Grade 100ml BUF-3024-1X100ml
500ml BUF-3024-1X500ml
0.1M EDTA Solution pH 7.4 Ultra Pure Grade 500ml BUF-1051-500ml-pH7.4
1L BUF-1051-1L-pH7.4
0.1M EDTA Solution pH 8.0 Ultra Pure Grade 500ml BUF-1051-500ml-pH8.0
1L BUF-1051-1L-pH8.0
0.5M EDTA Solution pH 8.0 Ultra Pure Grade 500ml BUF-1052-500ml-pH8.0
1L BUF-1052-1L-pH8.0
0.5M EDTA Solution pH 8.0 Biotechnology Grade 100ml BUF-1053-100ml-pH8.0
1L BUF-1053-1L-pH8.0
5.0M Sodium Chloride Solution Biotechnology Grade 500ml BUF-1112-500ml
1L BUF-1112-1L
1X SSC Buffer Biotechnology Grade 1L BUF-3050-1X1L
20X SSC Buffer Biotechnology Grade 1L BUF-3050-20X1L
20X SSC Buffer Ultra Pure Grade 1L BUF-3051-20X1L
1X SSPE Buffer Biotechnology Grade 1L BUF-3060-1X1L
20X SSPE Buffer Biotechnology Grade 1L BUF-3060-20X1L
Sterile Deionized Water Biotechnology Grade 1L BUF-4100-1L