The PrimeWay® Plasmid II DNA Extraction Kit is an improved version of our original kit, delivering higher yield and remains high purity.
Using the proven alkaline lysis method, it efficiently extracts high-quality plasmid DNA from bacteria with ease. The purified plasmid is ready for a wide range of downstream applications, including DNA sequencing, PCR, restriction mapping, cloning, and DNA labeling.
FEATURES
- High Purity – Extracted plasmid DNA consistently achieved OD260/280nm ≥ 1.8
- Maximized Recovery – Reliable yield, even from the most challenging vectors
- Higher Capacity – Process cell culture volumes up to 7 mL with ease
- Enhanced Performance – Preheated buffer boosts recovery of large plasmids
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APPLICATION DATA
Consistency of Purity Performance
The three graphs demonstrate that PrimeWay® Plasmid II DNA Extraction Kit (KIT-9041) delivers efficient extraction of high-quality plasmid DNA from diverse sample types. Consistently high purity of A260/280 and A260/230 ratios confirm minimal contamination and excellent DNA integrity, which is ideal for sensitive downstream applications including restriction digestion, cloning, and qPCR.
Recovery for Low, Medium
& High Copy Plasmid
Culture with low, medium and high copy number plasmid are extracted with PrimeWay® Plasmid II DNA Extraction Kit (KIT-9041). High-quality pure plasmid DNA recovery across all sizes and ready for downstream applications.
Optimal Recovery for
Various Plasmid Sizes
Plasmids of various sizes, ranging from 3 kb to 12 kb, purified with PrimeWay® Plasmid II DNA Extraction Kit (KIT-9041), were analyzed on a 1% TAE agarose gel. Extracted plasmid DNA quality is on par with leading brands.
Enhanced Input Capacity
Overnight cultures of E. coli 10β containing a high copy plasmid (pJET-BRCA) and a low copy plasmid (pBAD-HisA), respectively were prepared in varying culture volumes and extracted using the PrimeWay® Plasmid II DNA Extraction Kit (KIT-9041). Extracted plasmids were eluted in 100 µL of elution buffer. For both high-copy and low-copy plasmids, the PrimeWay® Plasmid II DNA Extraction Kit (KIT-9041) features a high-capacity binding column that processes up to 7 mL of culture in a single prep; ensuring efficient recovery and higher yields, making it ideal for high-output plasmid preparations.
High Quality DNA
Preheating the elution buffer to 70 °C significantly increases plasmid DNA yield compared to room-temperature elution, while maintaining high purity (A260/280). This approach can enhance total DNA recovery by up to 25%.
Competitor Data – Proven Yield & Purity
From 5 mL overnight cultures of various high-copy, medium-copy and low-copy number plasmids, multiple brands were tested side-by-side. Using each manufacturer’s protocol and 100 µL elution volume, PrimeWay® Plasmid II DNA Extraction Kit (KIT-9041) consistently delivered high-purity, high-yield results across plasmid sizes.
| Product Code | KIT-9041 |
| Product | PrimeWay® Plasmid II DNA Extraction Kit |
| Size | 10/50/250 prep |
| Sample Type | 1 to 7 mL Bacteria Culture |
| Yield | Up to 40 µg |
| Plasmid Size | Up to 15 kb |
| Binding Capacity | 60 µg |
| Elution | 50 to 100 µL |
| Duration | <25 min |
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| Size (preps) | Downloads | |
| Safety Data Sheet | 10 |  |
| 50 | |
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| 250 | |
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| Full Product Protocol | |
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| Product Brochure | |
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| Product Profile | |
