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Floro+Red Nucleic Acid Stain

0.6% Agarose. Precast gel staining of ExactMark 1kb DNA Ladder (BIO-5140) loaded in various volumes, 1X TAE Buffer (BUF-3000), 5v/cm for 45mins. Visualisation under standard UV transilluminator.

Product Series: BIO-5171

Excellent Sensitivity

    • Achieve bright, clear and defined bands across different fragment sizes

Flexible Use

    • Applicable to: dsDNA, ssDNA and RNA
    • Precast or post-gel staining protocols
    • Agarose and polyacrylamide gels

Safe For You

    • Non-mutagenic and non-cytotoxic as shown by Ames and Cell Staining Tests

Easy Disposal

    • Environmentally-friendly. Dispose as regular trash or down the drain**Institutional regulations may vary.

Floro+Green Nucleic Acid Stain

0.6% Agarose. Precast gel staining of ExactMark 1kb DNA Ladder (BIO-5140) loaded in various volumes, 1X TAE Buffer (BUF-3000), 5v/cm for 45mins. Visualisation under BLooK (blue light LED transilluminator).

Product Series: BIO-5172

Excellent Sensitivity

    • Achieve bright, clear and defined bands across different fragment sizes

Flexible Use

    • Applicable to: dsDNA, ssDNA and RNA
    • Precast or post-gel staining protocols
    • Agarose and polyacrylamide gels

Safe For You

    • Non-mutagenic and non-cytotoxic as shown by Ames and Cell Staining Tests

Easy Disposal

    • Environmentally-friendly. Dispose as regular trash or down the drain**Institutional regulations may vary.

FloroSafe DNA Stain

Product Series: BIO-5170, 1mL

Non-carcinogenic alternative of Ethidium Bromide, DNA Stain can be used as a safer alternative compared to the traditional ethidium bromide stain used for DNA and RNA detection in agarose gel. Compared to ethidium bromide, it has low toxicity and mutagenicity. DNA Stain has two secondary fluorescence excitation peaks (~270 nm; ~295 nm) and one strong excitation peak centered around 490 nm. The fluorescence emission is similar to Ethidium Bromide when bound to DNA which is at about 530 nm.

Usage Protocols

Precasting method:

    • Prepare 100ml of 0.8-3.0% agarose gel solution.
    • Heat till solution is completely clear.
    • Add 4-6µl of DNA stain and mix gently with gel.
    • Cool gel to 50-60°C and cast gel into gel tray.
    • Load samples into solidified gel and perform electrophoresis.
    • Detect bands under UV illuminator.

Post-staining method:

    • For agarose gel less than 0.5cm thickness, 10 – 25µl of the stain should be used per 100ml of buffer
    • Staining time is optimized between 5 – 60 minutes; amount of stain varies with gel thickness and concentration of agarose.
    • Visualize DNA by staining with DNA stain (C/No: BIO-5170-1ml).

Got Questions? Click Here for FAQs

Tri-Color 6X DNA Loading Dye

Product Series: BIO-5160

Used for loading DNA markers and samples on agarose or polyacrylamide gels. It contains three dyes – Orange G, Xylene cyanol FF and Bromophenol blue. This allows better visual tracking of DNA migration during electrophoresis.

6X DNA Loading Dye 

0.15% Orange G36% Glycerol
0.03% Bromophenol blue10Mm Tris-HCl (pH 7.4)
0.03% Xylene cyanol FF50mM EDTA (pH 8.0)

Protocol for Loading

    • Mix 1 part of dye solution with 5 parts of DNA sample.

Interested to try it out?

Product Description No of Applications Product No. Product Manual Safety Data Sheet
FloroSafe DNA Stain, 1ml ~200 applications (based on precasting method) BIO-5170-1ml
Floro+ Red Nucleic Acid Stain, 600ul ~200 applications (based on precasting method) BIO-5171-600ul
Floro+ Green Nucleic Acid Stain, 600ul ~200 applications (based on precasting method) BIO-5172-600ul
Tri-Color 6X DNA Loading Dye, 6 X 1ml 6000 applications BIO-5160-6ml
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Product Description Grade Packaging Product No. Product Specification Sheet Safety Data Sheet
Agarose Biotechnology Grade 100g BIO-1000-100g
500g BIO-1000-500g
1kg BIO-1000-1kg
Water DEPC treated Biotechnology Grade 200ml BUF-1170-200ml
1L BUF-1170-1L
Water Biotechnology Grade 100ml BUF-1180-100ml
500ml BUF-1180-500ml
1L BUF-1180-1L
1X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Ultra Pure Grade 1L BUF-3000-1X1L
10X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Ultra Pure Grade 1L BUF-3000-10X1L
4L BUF-3000-10X4L
50X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Ultra Pure Grade 1L BUF-3000-50X1L
4L BUF-3000-50X4L
10X Tris-Acetate-EDTA (TAE) Buffer pH 7.8 Ultra Pure Grade 1L BUF-3001-10X1L
10X Tris-Acetate-EDTA (TAE) Buffer Premixed Powder Ultra Pure Grade 1L BUF-3002-10X1L
1X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Biotechnology Grade 1L BUF-3003-1X1L
10X Tris-Acetate-EDTA (TAE) Buffer pH 8.0 Biotechnology Grade 100ml BUF-3003-10X100ml
1L BUF-3003-10X1L
10X Tris-Acetate-EDTA (TAE) Buffer Premixed Powder (Sachet) Ultra Pure Grade 1L BUF-3009-10X1L
1X Tris-Borate-EDTA (TBE) Buffer pH 8.3 Ultra Pure Grade 1L BUF-3010-1X1L
10X Tris-Borate-EDTA (TBE) Buffer pH 8.3 Ultra Pure Grade 1L BUF-3010-10X1L
4L BUF-3010-10X4L
10X Tris-Borate-EDTA (TBE) Buffer Premixed Powder Ultra Pure Grade 1L BUF-3011-10X1L
10X Tris-Borate-EDTA (TBE) Buffer pH 8.3 Biotechnology Grade 100ml BUF-3013-10X100ml
500ml BUF-3013-10X500ml
1L BUF-3013-10X1L
10X Tris-Borate-EDTA (TBE) Buffer Premixed Powder (Sachet) Ultra Pure Grade 1L BUF-3019-10X1L
1X Tris-EDTA (TE) Buffer pH8.0 Ultra Pure Grade 1L BUF-3020-1X1L
10X Tris-EDTA (TE) Buffer pH 8.0 Ultra Pure Grade 1L BUF-3020-10X1L
4L BUF-3020-10X4L
1X Tris-EDTA (TE) Buffer with reduced EDTA pH 8.0 Ultra Pure Grade 1L BUF-3021-1X1L
10X Tris-EDTA (TE) Buffer with reduced EDTA pH 8.0 Ultra Pure Grade 1L BUF-3021-10X1L
1X Tris-EDTA (TE) Buffer pH 8.0 Biotechnology Grade 100ml BUF-3024-1X100ml
500ml BUF-3024-1X500ml
0.1M EDTA Solution pH 7.4 Ultra Pure Grade 500ml BUF-1051-500ml-pH7.4
1L BUF-1051-1L-pH7.4
0.1M EDTA Solution pH 8.0 Ultra Pure Grade 500ml BUF-1051-500ml-pH8.0
1L BUF-1051-1L-pH8.0
0.5M EDTA Solution pH 8.0 Ultra Pure Grade 500ml BUF-1052-500ml-pH8.0
1L BUF-1052-1L-pH8.0
0.5M EDTA Solution pH 8.0 Biotechnology Grade 100ml BUF-1053-100ml-pH8.0
1L BUF-1053-1L-pH8.0
5.0M Sodium Chloride Solution Biotechnology Grade 500ml BUF-1112-500ml
1L BUF-1112-1L
1X SSC Buffer Biotechnology Grade 1L BUF-3050-1X1L
20X SSC Buffer Biotechnology Grade 1L BUF-3050-20X1L
20X SSC Buffer Ultra Pure Grade 1L BUF-3051-20X1L
1X SSPE Buffer Biotechnology Grade 1L BUF-3060-1X1L
20X SSPE Buffer Biotechnology Grade 1L BUF-3060-20X1L
Sterile Deionized Water Biotechnology Grade 1L BUF-4100-1L