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1st BASE Taq II Polymerase and PCR Master Mix

Enhanced Taq polymerase that gives superior performance on fidelity, stability and specificity.

1st BASE Taq II polymerase is an enhanced Taq DNA polymerase optimized for routine PCR applications. The enzyme provides a higher sensitivity, yields and longer PCR amplification as compared to conventional Taq DNA polymerase. It is supplied with optimized Taq II Polymerase 10X buffer and 25mM MgCl2, minimising the need for optimisation.

Highlights

  • Robust amplification
  • Higher sensitivity as compared to conventional Taq
  • Amplification of targets up to 6kb from genomic DNA
  • High efficiency and PCR yields
  • Fast – developed to work under fast thermal cycling conditions

Polymerase for everyday applications

  • DNA sequencing
  • DNA labelling
  • PCR for cloning
  • PCR ampli­fication of DNA fragments up to 6kb
  • Routine PCR
  • Colony PCR

Specifications

Fidelity 1X
Amplification length Up to 6 kb
Overhang Sticky ends/ 3'-A
Polymerase Taq II DNA polymerase
Reaction speed 1min/kb, 30s/kb

Robust and efficient amplification

Reliable amplification from various sample type and amplification length. Improved yield with optimal buffer system. Amplification of long DNA up to 6kb

DNA fragments across different targets and length were amplified from
(1) plasmid with an expected amplicon size of 407bp
(2) bacteria sample with expected size of 1500bp
(3) human gDNA with expected size of 3495bp
(4) human gDNA with expected size of 5999bp.


Better Sensitivity

Taq II display a much higher sensitivity as compared to conventional Taq as well as other competitive polymerases.

(A) Amplification of a 470bp fragment from plasmid DNA on different amount of DNA templates starting from 1ng to 0.1ng is shown above. (B) Amplification of 1500bp fragment from bacteria DNA on different amount of DNA templates starting from 25ng to 1ng.

Faster processivity

Improved Taq II developed to work under fast thermal cycling conditions with consistency.

Amplification of 407bp DNA fragment from plasmid DNA using different Taq II formulated product:

(1) Taq II DNA polymerase
(2) REDiant II Taq DNA polymerase
(3) Taq II PCR master mix
(4) REDiant II PCR master mix, based on extensions at 30s/kb.

Product

Polymerase Mastermix
Taq II BIO-5111-500U
BIO-5111-2500U
BIO-5181-200
BIO-5181-1000

REDiant II DNA Polymerase & Master Mix

1st BASE REDiant II Polymerase and PCR Mastermix uses the enhanced Taq II polymerase and are formulated with density reagents and inert tracking dyes to enable easy visual control during PCR set-up and direct loading of the reaction product into the gel.

REDiant II PCR Master Mix is a premixed, ready to use solution which contains Taq DNA polymerase, dNTPs, MgCl2 and reaction buffer for amplification of DNA templates by PCR. This pre-mixed formulation saves time and reduces contamination by reducing the number of pipetting steps required for usual PCR set up. The mix is optimized for efficient and reproducible PCR. It also includes a red, inert tracking dye which migrates at the same rate as 1kb DNA fragment in a 1% agarose gel.

Highlights

  • Direct loading of PCR products onto agarose gel without addition of gel loading buffer
  • Visualizes the addition of polymerase into the PCR reaction mix and ensure complete mixing
  • Acts as tracking dye in gel electrophoresis
  • Red inert dye does not inhibit PCR

Polymerase for everyday applications

  • DNA sequencing
  • DNA labelling
  • PCR for cloning
  • PCR ampli­fication of DNA fragments up to 6kb
  • Routine PCR
  • Colony PCR

Specifications

Fidelity 1X
Amplification length Up to 6 kb
Overhang Sticky ends/ 3'-A
Polymerase Taq II DNA polymerase
Reaction speed 1min/kb, 30s/kb
Direct Loading to gel Yes, red tracking dye


Robust and efficient amplification

Reliable amplification from various sample type and amplification length. Improved yield with optimal buffer system. Amplification of long DNA up to 6kb

DNA fragments across different targets and length were amplified from
(1) plasmid with an expected amplicon size of 407bp
(2) bacteria sample with expected size of 1500bp
(3) human gDNA with expected size of 3495bp
(4) human gDNA with expected size of 5999bp.

Product

Polymerase Mastermix
REDiant II BIO-5116-500U
BIO-5116-2500U
BIO-5187-200
BIO-5187-1000

exTEN II PCR Master Mix

exTEN II is an unique premixed blend of Taq DNA polymerase, a proofing enzyme, dNTPs, MgCl2 and reaction buffer for amplification of DNA templates by PCR

With the addition of a 3’ 5’ exonuclease (proofreading) ability, the amplification efficiency is enhanced through a lower rate misincorporated nucleotides compared to just Taq DNA Polymerase alone. exTEN II PCR Master Mix produces higher yields and amplifies longer fragments up to 10kb. Most of the amplified DNA fragments have 3’A overhang, while a small percentage are blunt-end. This premix formulation saves times and reduces contamination by reducing the number of pipetting steps for PCR setup. exTEN 2X PCR Master Mix consists of a density reagent and 2 tracking dyes which migrates at the same rate as a 4000bp and 50bp DNA fragment in a 1% agarose gel

Highlights

  • Superior yield and robust amplification of DNA fragments up to 10kb
  • Greater sensitivity achieved with low concentration template DNA
  • Minimum pipetting steps required as it is formulated for easy preparation and direct loading which also reduces contamination & errors

Specification

Fidelity 1X
Amplification length Up to 10 kb
Overhang Mix of Sticky & Blunt ends
Polymerase Taq II DNA polymerase & Proof-reading enzyme
Reaction speed 1min/kb, 30s/kb

Superior Yield & Robust Amplification of DNA Fragments Up to 10kb

DNA Fragments across different targets from a human genomic DNA were amplified with exTEN II PCR Master Mix and other commercial polymerases. In comparison to the other polymerases, amplification of target at 13.6kb and beyond with exTEN II is still achievable with superior yield.

Better Sensitivity Achieved even in Low Concentration of DNA Template

Amplification of a 677bp fragment from human genomic DNA on different amount of DNA templates starting from 1ng to 0.1ng is shown above. exTEN II produces better results in terms of sensitivity among the other polymerases.

(A) Amplification of a 470bp fragment from plasmid DNA on different amount of DNA templates starting from 5ng to 0.1ng is shown above. (B) Amplification of 1500bp fragment from bacteria DNA on different amount of DNA templates starting from 25ng to 1ng.

Superior Performance

Amplification shows better yield and efficiency against conventional Taq. Comparable with high-fidelity polymerase.


Amplification of a 3495bp fragment from human genomic DNA using (1) exTEN II PCR Master Mix (2) conventional taq (3) Brand T (4) Brand P

Convenient & Ease of Handling

Shortened Preparation Time

exTEN II is a premix containing all necessary components including MgCl2, buffer, loading dyes & dNTPs in a single tube. It only requires addition of template, primers and water prior to PCR cycling. Direct loading of PCR products on to gel is performed without any additional steps thereafter.

Product

Product Name Product Code
exTEN II PCR Mastermix BIO-5188-200
BIO-5188-1000

Sample Request Form

Our new enhanced PCR reagents are available in 50U sample sizes. Complete the form below and get a free sample delivered to your lab.

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