Leveraging our expertise in DNA sequencing, we offer DNA barcoding services for pure isolates of bacterial, fungal, plant and animal samples. For DNA barcoding of mixed isolates or environmental samples, please refer to Metagenomic Sequencing (NGS-7000 Series) in our Next-Generation Sequencing.

Molecular methods will be employed to give more accurate and reproducible results and identifying the samples down to the genus level.

Appropriate controls are used at all levels to ensure the results are consistent and of the highest quality. All reports include the assembled sequences of your samples and the alignment of the top 10 hits to GenBank databases.

Features of 1st BASE DNA Barcoding Services:

  • Barcoding and identification services for isolated pure samples
  • Accurate and reproducible results
  • Fast turnaround time
  • Bidirectional sequencing prevents ambiguity in sequence
  • Sequences are matched against a standard database, or a database of choice
  • Comprehensive reports include sequences of sample, and the top 10 Hits of the BLAST results
  • Neighbour Joining (unrooted tree)
  • Additional analyses e.g. Phylogenetic analysis between samples is available at separate charges
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Our DNA barcoding services is coupled with sample preparation services, allowing you to get the results in the shortest turnaround time, so that you can focus on the data analysis and decide the next move of your research experiments.

Bacteria

DescriptionService
Bacterial DNA Barcoding (pure isolates), full-length of 16s rRNA (~1400bp).

  • Extraction of gDNA
  • PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database)
MBS-5002

Eukaryote

Description

Service

Eukaryote DNA Barcoding, 18s rRNA (~1800bp)

  • Extraction of gDNA
  • PCR amplification and Purification
  • Primer walking of Cloned DNA
  • Data analysis (BLAST to show the top 10 matches from database)

MBS-5006

 

Fish

Description

Service

Fish DNA Barcoding, COI (~700bp)

  • Extraction of gDNA
  • PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database).

MBS-5009

 

Fungi

DescriptionService
Fungal DNA Barcoding (pure isolates), ITS (~700bp)

  • Extraction of gDNA
  • PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database).
MBS-5004

 

Insect

Description

Service

Insect DNA Barcoding, COI (~700bp)

  • Extraction of gDNA
  • PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database).

MBS-5010

 

Plant

DescriptionService
Plant DNA Barcoding, rbcl (~600bp) or matK (~850bp)

  • Extraction of gDNA
  • PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database)
MBS-5003

 

Custom DNA Barcoding

 

DescriptionService
Primer sequence provided by customer. Price is according the number of sample(s) for each primer set.

  • Extraction of gDNA
  • PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database)
MBS-5007

 

 

For sample submission guidelines and instructions for packing of samples, please visit the "Downloads" tab.

Contact Us for Orders or Enquiries

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Sample Submission Guidelines for MBS
Packing of Samples