PrimeWay Gel Extraction/ PCR Purification Kit offers 2 applications in one kit. It is a simple procedure that uses a silica-based spin column to perform gel extraction or purify the DNA fragments from 65 bp to 1.5 kb within 20 minutes with a high recovery rate. This kit enables removal of primers, dNTPs, enzymes, salts, and short PCR products (< 65 bp). Thus, making it suitable for downstream processes such as DNA sequencing, PCR, in-vitro transcription, restriction mapping, cloning, and labelling applications.

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FEATURES

  • Fast - 10 min for PCR Purification; 20 min for Gel Extraction
  • High recovery - Up to 95% for gel extraction & PCR clean-up
  • Efficient - Removal of reaction contaminants including enzymes, proteins, primers dimers, dNTPs, and salts
  • Convenience - No calculation required for BD buffer amount if gel slice ≤300mg

Interested to try it out?

APPLICATION DATA

High Quality DNA is Compatible for Wide Variety of Downstream Applications

including DNA sequencing, PCR, in-vitro transcription, restriction mapping, cloning, and labeling applications.

Performance Review

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Comparison Data

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50 ng of PCR product are analyzed using 1% TAE gel stained with Floro+Red Nucleic Acid Stain (BIO-5171-600ul). ExactMark 1 kb DNA ladder (1st BASE, BIO-5140-50ug) is used as the size standard.

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40 µL of 100 bp DNA ladder (1st BASE, BIO-5130-50ug) are purified using different brands of Gel/PCR kit. DNA is eluted with 40 µL Elution Buffer.
4 µL of purified 100 bp DNA ladder are analyzed using 1.7% TAE gel stained with Floro+Red Nucleic Acid Stain (BIO-5171-600ul).

APPLICATIONS

  • Suitable for downstream processes such as DNA sequencing, PCR, in-vitro transcription, restriction mapping, cloning and labelling applications.
Interested to try it out?
Product Code KIT-9050
Product PrimeWay Gel Extraction – PCR Purification Kit
Size 10/50/250 prep
Sample Type Gel / PCR mixture
Yield/Recovery  Up to 95%
Purity 1.7 - 2.0
Duration  PCR Purification: 10; Gel Extraction: 20 min
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Size (preps) Downloads
Safety Data Sheet 10
50
250
Product Manual  
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