PrimeWay Viral DNA / RNA Extraction Kit is designed specifically for efficient purification of viral DNA and viral RNA from serum, plasma, body fluids, cell culture supernatant and viral transport medium swabs. The special feature of this kit is that it includes an inert carrier [poly-A] powder which enhances the binding of viral nucleic acid onto the silica membrane to improve its binding efficiency. It is an ideal kit to isolate low viral copies from 10E1 to 10E6 copies/mL of viral nucleic acid. The purified viral nucleic acid is optimum for qPCR, RT-PCR, RT-qPCR & Next Generation Sequencing applications. The end-user can purify their viral DNA/RNA in less than 30 minutes.
FEATURES
- Sensitive - Virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number.
- Low Sample Required - < 200µl plasma, serum, body fluid or the supernatant of viral infected cell cultures, nasopharyngeal and oropharyngeal swabs.
- Fast - Able to purify virus DNA or RNA in 20 minutes!
- Includes an inert carrier [poly-A] powder.
Interested to try it out?
APPLICATIONS
- Isolated nucleic acid is ideal for use in qPCR, RT-PCR, RT-qPCR & Next Generation Sequencing applications
Detection of SARS-CoV-2
Virus RNA was purified from 800 copy number of SARS-CoV-2 using PrimeWay Viral DNA/RNA Extraction Kit (KIT-9010) with 6 replicates. The purified RNA was eluted with 40 µL RNase-free water. A reverse transcription PCR assay was performed with 1 µL of extracted RNA. The result confirmed that virus RNA can be successfully extracted with 70 – 90% recovery rate. The average cycle threshold (Ct) is 35.60.
| Product Code | KIT-9010 |
| Product | PrimeWay Viral DNA/RNA Extraction Kit |
| Size | 10/50 prep |
| Sample Type | Serum, Plasma, Body Fluids, Cell Culture Supernatant and Viral Transport Medium Swabs |
| Yield/Recovery | < 1 ug |
| Duration | 30 min |
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| Size (preps) | Downloads | |
| Safety Data Sheet | 10 |  |
| 50 | |
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| Product Manual | |
