Your Plasmid. Fully Verified. Within 24 Hours.
Whole Plasmid Sequencing by 1st BASE delivers fast, accurate full plasmid sequencing using advanced long‑read technology.
Designed for researchers who need complete plasmid validation, our service provides end‑to‑end coverage, eliminates the need for primer design, and offers reliable results within 24 hours. Each run includes an annotated plasmid map, consensus sequence, raw reads, and a comprehensive QC report - giving you the confidence to move forward with cloning, vector design, gene therapy applications, and more.
Key Features
- Complete Plasmid Coverage - Verify your entire plasmid in a single sequencing run
- Fast Turnaround - Get your sequencing results within 24 hours.
- No Primer Design - Skip the hassle of designing multiple sequencing primers
- Ready-to-Use Results - Receive comprehensive sequencing data and analysis for confident plasmid verification
Everything you need. In one run.
- Full Plasmid Sequence - Complete sequence coverage for comprehensive plasmid analysis.
- Long-Read Data - Reads spanning several kilobases for structural and sequence analysis.
- PlasmidLab Access - Review and explore your sequencing results on the PlasmidLab bioinformatics platform.
- Multiple Data Formats - FASTQ • Consensus FASTA • Annotated GenBank • Plasmid Map • QC Report
Traditional Sanger Sequencing vs Whole Plasmid Sequencing
| Traditional Sanger Sequencing | Whole Plasmid Sequencing | |
|---|---|---|
| Coverage and Read Length | Usually one targeted region per reaction, 500–1,000 bp | Entire plasmid, including insert and backbone, reads can span several kilobases |
| Cost Effectiveness | Cheaper for checking one short region | Better value when complete-plasmid verification is required |
| Raw Data Output | Chromatogram and sequence file, such as .ab1 and FASTA | Long-read files such as FASTQ, plus assembly and annotation report |
| Structural Changes/Detection | Strong for SNVs and small indels within a clean, high-quality trace | Detects SNVs, indels and potentially larger structural changes such as rearrangements across the plasmid |
| Primers | One sequencing primer per reaction | No sequencing primer required |
Sample Submission Requirements
| Plasmid Size | Concentration (ng/µl) | Volume (µl) | Purity |
|---|---|---|---|
| ≤10 kb | ≥20 | 20 | (A₂₆₀/A₂₈₀) 1.8-2.0 (A₂₆₀/A₂₃₀) ≥2.0 |
| 10–20 kb | ≥30 | ||
| ≥20 kb | ≥40 |
- We require purified, intact, circular double stranded plasmid DNA.
- Plasmid DNA should be eluted in Elution Buffer or Nuclease Free Water.
- Submit Plasmid DNA in 1.5ml microcentrifuge tube.
- DNA quantification should be done prior to sample submission using fluorometric methods. (Spectrometric measurements tends to give a higher concertation reading as it is unable to distinguish between dsDNA and ssDNA)