Sanger sequencing method was developed by two-time Nobel Laureate Frederick Sanger and his colleagues in 1977, hence the technology was named as Sanger Sequencing.

1st BASE has been offering Sanger Sequencing services since year 2002. We started from the manual slab-gel electrophoresis system to the current automatic genetic analyzer. We had adopted the 96-capillary system, which is the highest capacity genetic analyzer from Applied Biosystems® with our optimized protocols to give you superior data in the shortest turnaround time. We are using the chain-terminator cycle sequencing chemistry, as its robust nature makes it highly suitable for a variety of applications and various type of pure DNA templates.

Be assured that your DNA template for Sanger sequencing is in good hands. We work hard to make sure we continuously meet or exceed leading industry providers in terms of quality value (QV), trace score and contiguous read length (CRL). Our experienced team ensures quality results even with difficult templates, and provide prompt troubleshooting assistance.

For DNA templates that contain little mixture of sequences, you may refer to our Molecular Biology Services which adopted DNA cloning before sequencing a few positive colonies.

For DNA templates that contain high mixture of sequences (e.g. PCR products amplified from environmental samples), you may refer to our amplicon sequencing services from Next-Generation Sequencing.