PCR Product Cloning
We offer PCR Product Cloning service to clone unpurified PCR product into pJET1.2/Blunt vectors. For PCR Product Cloning Services, screening of positive clones will be done and the positive clone containing full length insert will send for sequencing for sequence confirmation. We also offer additional screening and sequencing of up to 5 positive colonies with PCR Product Cloning Service PLUS services.
Minimum setup fee will apply when no positive clone obtained after picking of up to 8 clones with control. Additional screening of positive clones is also available at separate cost.
PCR Product Cloning Service (up to 1.5kb)
PCR Product Cloning Service PLUS (up to 1.5kb)
pJET1.2™ is the registered trademark from Life Technologies and Thermo Fisher Scientific.
Our subcloning service includes subcloning of an insert from its original holding plasmid or a gene synthesis product into the customer choice of destination vector, colony PCR screening (pick up to 24 colonies) and DNA sequencing for confirmation.
We will perform verification on quality and integrity upon receipt of original sample(s) before proceeding with any subcloning service.
Due to intellectual property rights, our subcloning services does not include the cost of destination vector. Instead, customers will need to provide us the destination vector separately upon order confirmation.
The final deliverables include the new gene construct as lyophilized DNA, together with its sequencing data and a service report.
Minimum setup fee will apply when no positive clone obtained after picking of up to 24 clones with control. Additional screening of positive clones is also available at separate cost.
Subcloning Service (up to 1.5kb of insert)
Fragment assembly joins multiple overlapping double stranded DNA fragments. In the end of the fragment assembly, the final construct will be cloned into a holding plasmid and sequence verified by Sanger sequencing.
This method is widely used during construction of longer genes, where synthesis of a long gene is chemically challenging and expensive.
Two services are offered as below:
- Assembly of IDT gBlocks™ into pJET1.2™/ Blunt vector
- This includes fragments design and assembly, cloning and sequencing of the final construct.
- This includes fragments design and assembly, primer synthesis, PCR, cloning and sequencing of the final construct.
Fragment Assembly. Number of fragments = x.
gBlocks™ is the registered trademark from Integrated DNA Technologies (IDT)
Site-Directed Mutagenesis (SDM)
Site-Directed Mutagenesis (SDM) is a method to create specific changes in a double stranded plasmid DNA. It uses combination of techniques from DNA synthesis and advanced-PCR. Each mutation site is defined as any combination of mutation within the frame of 50 bases. The mutation may be a single base change, multiple base changes, insertion or deletion.
Site-directed Mutagenesis (SDM)
· Verification of insert gene
· Design and synthesis of PCR primers
· Mutagenesis of target gene
· Sequencing verification up to 1.5kb
DNA Cloning - Additional Services
Minimum setup fee will apply when no positive clone obtained after picking of up to 24 clones with control.
Additional screening for positive clone. Price per screen (24 colony picked).
Primer Walking of Constructs Service - Single Pass
Surcharge for gene construct > 1.5kb. Price per base.