PCR Product Cloning

We offer PCR Product Cloning service to clone unpurified PCR product into pJET1.2/Blunt vectors. For PCR Product Cloning Services, screening of positive clones will be done and the positive clone containing full length insert will send for sequencing for sequence confirmation. We also offer additional screening and sequencing of up to 5 positive colonies with PCR Product Cloning Service PLUS services.

Minimum setup fee will apply when no positive clone obtained after picking of up to 8 clones with control. Additional screening of positive clones is also available at separate cost.

Description

Service

PCR Product Cloning Service (up to 1.5kb)
Includes: Cloning of unpurified PCR product into pJET1.2™/ Blunt vector, colony PCR screening and DNA sequencing for confirmation.

MBS-3002 

PCR Product Cloning Service PLUS (up to 1.5kb)
Includes: Cloning of unpurified PCR product into pJET1.2™/ Blunt vector, colony PCR screening and pick 5 positive colony PCR products for sequencing (1xFor & 1xRev) confirmation.

MBS-3006

pJET1.2™ is the registered trademark from Life Technologies and Thermo Fisher Scientific.

Subcloning Service

Our subcloning service includes subcloning of an insert from its original holding plasmid or a gene synthesis product into the customer choice of destination vector, colony PCR screening (pick up to 24 colonies) and DNA sequencing for confirmation.

We will perform verification on quality and integrity upon receipt of original sample(s) before proceeding with any subcloning service.

Due to intellectual property rights, our subcloning services does not include the cost of destination vector. Instead, customers will need to provide us the destination vector separately upon order confirmation.

The final deliverables include the new gene construct as lyophilized DNA, together with its sequencing data and a service report.

Minimum setup fee will apply when no positive clone obtained after picking of up to 24 clones with control. Additional screening of positive clones is also available at separate cost.

 

Description

Service

Subcloning Service (up to 1.5kb of insert)
Includes: Subcloning of an insert from its original holding plasmid into other destination vector, colony PCR screening and DNA sequencing for confirmation.

MBS-3001 

Fragment Assembly

Fragment assembly joins multiple overlapping double stranded DNA fragments. In the end of the fragment assembly, the final construct will be cloned into a holding plasmid and sequence verified by Sanger sequencing.

This method is widely used during construction of longer genes, where synthesis of a long gene is chemically challenging and expensive.

Two services are offered as below:

  1. Assembly of IDT gBlocks™ into pJET1.2™/ Blunt vector
    • This includes fragments design and assembly, cloning and sequencing of the final construct.
  2. Assembly of dsDNA fragments (PCR products and synthetic genes)
    • This includes fragments design and assembly, primer synthesis, PCR, cloning and sequencing of the final construct.

 Description

Service

Fragment Assembly. Number of fragments = x. 

  • Fragment Assembly
  • Cloning
  • Sequencing of insert up to 1.5kb

MBS-8004 (N=x) 

gBlocks™ is the registered trademark from Integrated DNA Technologies (IDT)

Site-Directed Mutagenesis (SDM)

Site-Directed Mutagenesis (SDM) is a method to create specific changes in a double stranded plasmid DNA. It uses combination of techniques from DNA synthesis and advanced-PCR. Each mutation site is defined as any combination of mutation within the frame of 50 bases. The mutation may be a single base change, multiple base changes, insertion or deletion.

 

Description

Service

Site-directed Mutagenesis (SDM)

Includes:

·        Verification of insert gene

·        Design and synthesis of PCR primers

·        Mutagenesis of target gene

·        Subcloning

·        Sequencing verification up to 1.5kb

MBS-8006

 

DNA Cloning - Additional Services

Minimum setup fee will apply when no positive clone obtained after picking of up to 24 clones with control.

Additional screening for positive clone. Price per screen (24 colony picked).

MBS-3005

Primer Walking of Constructs Service - Single Pass

Surcharge for gene construct > 1.5kb. Price per base.

SS1008

 

Step 1: Download Order Form

Download and complete 1st BASE MBS Order Form (under Downloads tab) with your order requirements.

Step 2: Email the form to our email address below:

Singapore
Email: mbs@axilscientific.com
Tel: +65 6775 7318

Malaysia / International
Email: mbs@apicalscientific.com
Tel: +603 8943 3252

For customers who ship or courier us the orders directly, please indicate your airway bill number or courier tracking number in your email to ensure speedy processing of your orders.

Order Forms Singapore Malaysia/International
Sample Preparation Services
PCR Services
Cloning and Synthetic Biology
Genotyping Services
DNA Barcoding Services
Site-directed Mutagenesis (SDM)