The general workflow of NGS include sample preparation, original sample Quality Control (QC), library construction, library Quality Control (QC), sequencing run and bioinformatics analysis. 1st BASE offers NGS services in several categories to meet different needs of customers. They are: -

Standard NGS Services with/ without Bioinformatics Analysis

Customer provides purified nucleic acid that meets our sample requirements. 1st BASE performs original sample quality control (QC), library construction, library QC, sequencing run with or without bioinformatics analysis.

Name of ServiceProduct No.
Whole Genome Sequencing (WGS)
*For human, animal, plant, fungi, bacterium, virus or insect
NGS-4000 Series
Whole Exome Sequencing
*For human or mouse
NGS-5000 Series
Transcriptome Sequencing
*For mRNA, small RNA, lnRNA or Iso-Seq
NGS-6000 Series
Metagenomic Sequencing
*For environmental samples to target shotgun metagenome, virome, meta-transcriptomic, meta-barcoding, eDNA or any specific region of 16s/ ITS/ 18s gene in amplicon sequencing
NGS-7000 Series
Epigenome Sequencing
*For Human Whole Genome Bisulfite Sequencing (WGBS) or ChIP (Chromatin Immuno Precipitation) Sequencing
NGS-8000 Series
Other Applications
*Not limited to but includes custom target capture sequencing, RAD sequencing, organelle sequencing (mtDNA, chloroplast, plasmid), deep amplicon sequencing, fosmid sequencing or BAC sequencing.
NGS-9000 Series

Sample Preparation and Quantification Service

Customer provides raw materials that meets our sample submission guidelines. 1st BASE performs nucleic acid extraction, purification, sample quality control (QC) with or without library construction services.

Name of ServiceService DescriptionProduct No.
NGS Grade DNA Extraction1st BASE to perform DNA extraction, purification, and DNA sample QC. The DNA amount for each order may varies according to different library construction or nature of the project.NGS-1001
NGS Grade RNA Extraction1st BASE to perform total RNA extraction, purification, and RNA sample QC. The RNA amount or RIN requirements for each order may varies according to different library construction or nature of the project.NGS-1002
Sample QCOriginal Sample QC and report.NGS-1003
DNA PurificationRNase treatment follow by DNA purification.NGS-1004
RNA PurificationDNase treatment follow by total RNA purification.NGS-1005
DNA Extraction from Soil/ Stool/ Sludge SamplesCustomer to provide raw sample according to our submission guideline. 1st BASE to perform total DNA extraction and purification. The extracted DNA is only suitable for NGS-7000 series of Amplicon Sequencing.NGS-1007
Custom Primer Synthesis for Amplicon SequencingCustomer to send amplicons (<500bp) and provide custom primer sequences. Charge per Primer Set to enable Illumina custom amplicon library preparation.NGS-1008
Library QCLibrary QC by qPCR and DNA Fragment Analysis by Agilent TapeStation/ High Sensitivity DNA Labchip/ any equivalent.NGS-2001
Library Preparation - Illumina platform (Kit dependent)Library Preparation and with Library Quantification/ Library QC.NGS-2002
Library Preparation - PacBio platformSequel II CLR/ HiFi 10kb Library Preparation and Library QC.NGS-2003
Library PoolingEqual molar pooling of different libraries to run in the single sequencing flow cell.NGS-2004

NGS Custom Run Services

Customer provides self-prepared NGS libraries that meets our sample submission guidelines. 1st BASE performs sequencing run with or without library QC services. The sequencing raw data from Illumina MiSeq runs will be provided in Illumina BaseSpace as default with free of charge. *To sign up for a BaseSpace account and retrieving your NGS data from within, please click on the following link for a step by step guide: https://base-asia.com/downloads/products/NGS/Guidelines-for-downloading-NGS-data-from-Illumina-BaseSpace-v2.pdf. The raw data from other sequencing platforms can be provided in Ali Cloud, Amazon Web Services (AWS), Flash Drive or External Hard Disk with separate charges.

Name of ServiceService DescriptionProduct No.
Illumina Single Read Sequencing
    • Illumina NovaSeq 50 SE
    • Minimum of 1 million read per library
    • Raw data only
NGS-3001
Illumina NovaSeq Sequencing (Per Gb)
    • Illumina NovaSeq 6000
    • 150 PE run ü  Minimum of 1Gb
    • Raw data only
NGS-3003
Illumina NovaSeq Sequencing (Per Lane)
    • Illumina NovaSeq 6000
    • 150 PE run
    • Minimum of 850Gb per lane
    • Raw data only
NGS-3004
PacBio Sequel II Sequencing (per Gb)
    • PacBio Sequel II CLR run mode
    • Minimum 30Gb for each library
    • Raw data only
NGS-3005
PacBio Sequel II Sequencing (per Cell)
    • PacBio Sequel II CLR run mode (>=80Gb per cell);
      Or PacBio Sequel II CCS run mode (>=280Gb per cell)
    • Raw data only
NGS-3006
Illumina MiSeq Sequencing v2 (25 PE)
    • Illumina MiSeq 25 PE run, 1 flow cell
    • PhiX Spike-in 30%, unless advised by customer
    • Reagent Kit v2 (MS-102-2001) to deliver 12-15 Million PE reads per flow cell
    • Data output is subject to final PhiX spike-in amount and nature of pre-made libraries: ~750Mb to 850Mb per flow cell
    • Raw data only
NGS-3007
Illumina MiSeq Sequencing v2 (150 PE) Nano Output
    • Illumina MiSeq 150 PE run, 1 flow cell
    • PhiX Spike-in 30%, unless advised by customer
    • Reagent Kit v2 (MS-103-1001) to deliver 1 million PE reads per flow cell
    • Data output is subject to final PhiX spike-in amount and nature of pre-made libraries: ~300Mb per flow cell
    • Raw data only
NGS-3010
Illumina MiSeq Sequencing v2 (250 PE) Nano Output
    • Illumina MiSeq 250 PE run, 1 flow cell
    • PhiX Spike-in 30%, unless advised by customer
    • Reagent Kit v2 (MS-103-1003) to deliver 1 million PE reads per flow cell
    • Data output is subject to final PhiX spike-in amount and nature of pre-made libraries: ~500Mb per flow cell
    • Raw data only
NGS-3012
Illumina MiSeq Sequencing v3 (75 PE)
    • Illumina MiSeq 75 PE run, 1 flow cell
    • PhiX Spike-in 30%, unless advised by customer
    • Reagent Kit v3 (MS-102-3001) to deliver 22-25 Million PE reads per flow cell
    • Data output is subject to final PhiX spike-in amount and nature of pre-made libraries: ~3Gb per flow cell
    • Raw data only
NGS-3013
Illumina MiSeq Sequencing v3 (300 PE)
    • Illumina MiSeq 300 PE run, 1 flow cell
    • PhiX Spike-in 30%, unless advised by customer
    • Reagent Kit v3 (MS-102-3003) to deliver 22-25 Million PE reads per flow cell
    • Data output is subject to final PhiX spike-in amount and nature of pre-made libraries: ~13Gb to 15Gb per flow cell for WGS/ ~7Gb to 9Gb per flow cell for custom amplicon sequencing
    • Raw data only
NGS-3014
Nanopore MinION/ GridION Sequencing
    • Nanopore run in 1x Flow Cell (R9.4.1)
    • Flow Cell Kit (FLO-MIN106D) to deliver up to 20Gb per flow cell
    • Data output is subject to sample and preparation methods
    • Raw data only
NGS-3015
Data DeliveryIn Illumina BaseSpace/ FTP Link/ Ali Cloud/ AWS/ Flash Drive/ External Hard DiskNGS-DATA

Step 1: Download Order Form

Download and complete Order Form (under Downloads tab) with your order requirements.

For the source of sample(s) derived from inactivated pathogenic agents, we will need you to fill-up Biosafety Declaration Form (from Download Tab) to confirm the acceptance of your Order.

For NGS order requires Standard Bioinformatics Analysis, we will email you the respective Bioinformatics (BI) Analysis Form separately. We need the BI Analysis Form to reach us before the sequencing raw data is available to avoid delays in releasing the results.


Step 2: Email
 the form to our email address below:

Singapore
Email: ngs@axilscientific.com
Tel: +65 6775 7318

Malaysia / International
Email: ngs@apicalscientific.com
Tel: +603 8943 3252

For customers who ship or courier us the orders directly, please indicate your airway bill number or courier tracking number in your email to ensure speedy processing of your orders.


Click below for Guides on Preparations and Requirements

Download
Technical Bulletin
Biosafety Declaration Form

 

What our Customers Say about our NGS Services:

"We tried a couple of NGS service providers and eventually chosen Axil Scientific as our long-term service provider. The turnaround time for the service is fast (5-7 working days). In general, we are quite satisfied with the service and the quality of the data."

Simon Ng, A*STAR



"The team members from Axil Scientific had generously awarded us with an amazing technical and scientific supports as a one-stop solution for all our needs from library preparation to target identifications and personalized bioinformatics solutions. The team had helped us to prepare for the highest quality transcriptome sequencing data from the beginning of workflow, and offered effective steps to the troubleshooting at all stages. The bioinformatics solutions are of best quality covering all computationally intensive stages from reads mapping, transcripts alignments and downstream discovery analysis. I am amazed with the resultant output datasets which are very comprehensive, listing all detailed statistical data generated at each step. Given the complexity of getting pure subpopulations from cancer cells after undergoing fluorescence-activated cell sorting, we succeeded to achieve good QC and able to sequence with alignment rate of over 97% from low input RNA samples. Looking at the high quality and vast amount of data we have generated in short period of time, this has given us a unique opportunity and advantage to speed up the analysis. Time is always the limiting factor in science and by receiving supports from group of experts in Axil, we definitely find better solutions. Thank you very much to Axil Scientific for the NGS service award given and for supporting our research."

Siti Sarah Daud, NUS



“Axil provide a very good NGS service, the whole 16s rDNA sequencing takes only about 2 weeks, which is very fast and helpful to my research. The quality of their NGS result is good for the following bioinformatics analysis. Besides, the product specialist, Mr. Yu Tiam Meng, is a very nice and polite man, and he is one of the best product specialist I have even met in Singapore.”

Yang Yi, NUS



“It was a pleasure working with 1st base. They were fast and responded quickly to our inquiries and efficient in obtaining the samples, generating libraries and sequencing our RNA. Their customer service was good with clear communications and the data was easy to access. We are still analysing our data but so far it seems to be of high quality and sufficient coverage for our purposes. We look forward to working with them in the future.”

Aaron Irving, DUKE-NUS



“The service that I got from 1st BASE was excellent in every respect.”

Md. Monjurul Alam, Bangladesh Jute Research Institute