The general workflow of NGS include sample preparation, original sample Quality Control (QC), library construction, library Quality Control (QC), sequencing run and bioinformatics analysis. 1st BASE offers NGS services in several categories to meet different needs of customers. They are: -

Standard NGS Services with/ without Bioinformatics Analysis

Customer provides purified nucleic acid that meets our sample requirements. 1st BASE performs original sample quality control (QC), library construction, library QC, sequencing run with or without bioinformatics analysis.

Name of ServiceProduct No.
Whole Genome Sequencing (WGS)
*For human, animal, plant, fungi, bacterium, virus or insect
NGS-4000 Series
Whole Exome Sequencing
*For human or mouse
NGS-5000 Series
Transcriptome Sequencing
*For mRNA, small RNA, lnRNA or Iso-Seq
NGS-6000 Series
Metagenomic Sequencing
*For environmental samples to target shotgun metagenome, virome, meta-transcriptomic, meta-barcoding, eDNA or any specific region of 16s/ ITS/ 18s gene in amplicon sequencing
NGS-7000 Series
Epigenome Sequencing
*For Human Whole Genome Bisulfite Sequencing (WGBS) or ChIP (Chromatin Immuno Precipitation) Sequencing
NGS-8000 Series
Other Applications
*Not limited to but includes custom target capture sequencing, RAD sequencing, organelle sequencing (mtDNA, chloroplast, plasmid), deep amplicon sequencing, fosmid sequencing or BAC sequencing.
NGS-9000 Series

Sample Preparation and Quantification Service

Customer provides raw materials that meets our sample submission guidelines. 1st BASE performs nucleic acid extraction, purification, sample quality control (QC) with or without library construction services.

Name of ServiceService DescriptionProduct No.
NGS Grade DNA Extraction1st BASE to perform DNA extraction, purification, and DNA sample QC. The DNA amount for each order may varies according to different library construction or nature of the project.NGS-1001
NGS Grade RNA Extraction1st BASE to perform total RNA extraction, purification, and RNA sample QC. The RNA amount or RIN requirements for each order may varies according to different library construction or nature of the project.NGS-1002
Sample QCOriginal Sample QC and report.NGS-1003
DNA PurificationRNase treatment follow by DNA purification.NGS-1004
RNA PurificationDNase treatment follow by total RNA purification.NGS-1005
DNA Extraction from Soil/ Stool/ Sludge SamplesCustomer to provide raw sample according to our submission guideline. 1st BASE to perform total DNA extraction and purification. The extracted DNA is only suitable for NGS-7000 series of Amplicon Sequencing.NGS-1007
Custom Primer Synthesis for Amplicon SequencingCustomer to send amplicons (<500bp) and provide custom primer sequences. Charge per Primer Set to enable Illumina custom amplicon library preparation.NGS-1008
Library QCLibrary QC by qPCR and DNA Fragment Analysis by Agilent TapeStation/ High Sensitivity DNA Labchip/ any equivalent.NGS-2001
Library Preparation - Illumina platform (Kit dependent)Library Preparation and with Library Quantification/ Library QC.NGS-2002
Library Preparation - PacBio platformSequel II CLR/ HiFi 10kb Library Preparation and Library QC.NGS-2003
Library PoolingEqual molar pooling of different libraries to run in the single sequencing flow cell.NGS-2004

NGS Custom Run Services

Customer provides self-prepared NGS libraries that meets our sample submission guidelines. 1st BASE performs sequencing run with or without library QC services. The sequencing raw data from Illumina MiSeq runs will be provided in Illumina BaseSpace as default with free of charge. *To sign up for a BaseSpace account and retrieving your NGS data from within, please click on the following link for a step by step guide: https://base-asia.com/downloads/products/NGS/Guidelines-for-downloading-NGS-data-from-Illumina-BaseSpace-v2.pdf. The raw data from other sequencing platforms can be provided in Ali Cloud, Amazon Web Services (AWS), Flash Drive or External Hard Disk with separate charges.

Name of ServiceService DescriptionProduct No.
Illumina Single Read Sequencing
    • Illumina NovaSeq 50 SE
    • Minimum of 1 million read per library
    • Raw data only
NGS-3001
Illumina NovaSeq Sequencing (Per Gb)
    • Illumina NovaSeq 6000
    • 150 PE run ü  Minimum of 1Gb
    • Raw data only
NGS-3003
Illumina NovaSeq Sequencing (Per Lane)
    • Illumina NovaSeq 6000
    • 150 PE run
    • Minimum of 850Gb per lane
    • Raw data only
NGS-3004
PacBio Sequel II Sequencing (per Gb)
    • PacBio Sequel II CLR run mode
    • Minimum 30Gb for each library
    • Raw data only
NGS-3005
PacBio Sequel II Sequencing (per Cell)
    • PacBio Sequel II CLR run mode (>=80Gb per cell);
      Or PacBio Sequel II CCS run mode (>=280Gb per cell)
    • Raw data only
NGS-3006
Illumina MiSeq Sequencing v2 (25 PE)
    • Illumina MiSeq 25 PE run, 1 flow cell
    • PhiX Spike-in 30%, unless advised by customer
    • Reagent Kit v2 (MS-102-2001) to deliver 12-15 Million PE reads per flow cell
    • Data output is subject to final PhiX spike-in amount and nature of pre-made libraries: ~750Mb to 850Mb per flow cell
    • Raw data only
NGS-3007
Illumina MiSeq Sequencing v2 (150 PE) Nano Output
    • Illumina MiSeq 150 PE run, 1 flow cell
    • PhiX Spike-in 30%, unless advised by customer
    • Reagent Kit v2 (MS-103-1001) to deliver 1 million PE reads per flow cell
    • Data output is subject to final PhiX spike-in amount and nature of pre-made libraries: ~300Mb per flow cell
    • Raw data only
NGS-3010
Illumina MiSeq Sequencing v2 (250 PE) Nano Output
    • Illumina MiSeq 250 PE run, 1 flow cell
    • PhiX Spike-in 30%, unless advised by customer
    • Reagent Kit v2 (MS-103-1003) to deliver 1 million PE reads per flow cell
    • Data output is subject to final PhiX spike-in amount and nature of pre-made libraries: ~500Mb per flow cell
    • Raw data only
NGS-3012
Illumina MiSeq Sequencing v3 (75 PE)
    • Illumina MiSeq 75 PE run, 1 flow cell
    • PhiX Spike-in 30%, unless advised by customer
    • Reagent Kit v3 (MS-102-3001) to deliver 22-25 Million PE reads per flow cell
    • Data output is subject to final PhiX spike-in amount and nature of pre-made libraries: ~3Gb per flow cell
    • Raw data only
NGS-3013
Illumina MiSeq Sequencing v3 (300 PE)
    • Illumina MiSeq 300 PE run, 1 flow cell
    • PhiX Spike-in 30%, unless advised by customer
    • Reagent Kit v3 (MS-102-3003) to deliver 22-25 Million PE reads per flow cell
    • Data output is subject to final PhiX spike-in amount and nature of pre-made libraries: ~13Gb to 15Gb per flow cell for WGS/ ~7Gb to 9Gb per flow cell for custom amplicon sequencing
    • Raw data only
NGS-3014
Nanopore MinION/ GridION Sequencing
    • Nanopore run in 1x Flow Cell (R9.4.1)
    • Flow Cell Kit (FLO-MIN106D) to deliver up to 20Gb per flow cell
    • Data output is subject to sample and preparation methods
    • Raw data only
NGS-3015
Data DeliveryIn Illumina BaseSpace/ FTP Link/ Ali Cloud/ AWS/ Flash Drive/ External Hard DiskNGS-DATA
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Sample Submission Guidelines for gDNA/RNA Extraction Projects