At 1st BASE, we have the in-house expertise in processing samples for downstream molecular biology applications routinely. Researchers may depend on us to process their samples for their processes without fuss. 

The process of sample preparation is tedious and the amount of samples are typically limited for research. To maximise success for each sample preparation service, we advise our customers on how to collect and submit the samples within the acceptable terms and conditions. Such information is also available in each of the respective order forms. Minimum setup fee will apply when sample preparation service fails due to bad quality of sample that did not meet the prescribed terms and conditions.

DNA Extraction and Preparation Services

Service TypeDescriptionService
DNA analysisDNA Analysis for dsDNA(35bp-1000bp) by automated gel electrophoresisMBS-6007
DNA Quantification - By Agarose Gel/ By SpectrophotometerSS1020/SS1021
Other DNA servicesDNA digestion using Restriction Enzyme (RE), up to 2 RE.MBS-6002
DNA shearing by sonication and PCR clean-upMBS-6005
Blunting and phosphorylation of dsDNA (100-2500bp) and PCR clean-upMBS-6006
Purification of Genomic DNA (gDNA) to remove impurities that inhibits PCR.MBS-6008

Sample Submission Guidelines for gDNA/RNA Extraction Projects

RNA Extraction and Analysis

Service TypeDescriptionService
RNA extractionStandard total RNA extraction from up to 100mg of starting material. From various sources: Cells, tissues, blood, saliva, insect, fungi, bacteria, plant leaves, plant fruits, plant seeds, soil etc.MBS-6103
RNA analysisRNA LabChip Analysis Total RNA (25 to 500 ng/uL), Eukaryotic, 18S and 28S, size 25 - 6000 bp.MBS-6100
RNA LabChip Analysis Total RNA (25 to 500 ng/uL), Prokaryotic, 16S and 23S, size 25 - 6000 bp.MBS-6101
RNA LabChip Analysis Total RNA (25 to 500 ng/uL), Eukaryotic (Plant), 18S and 25S, size 25 - 6000 bp.MBS-6102
Other RNA servicesRNA Preparation Reverse Transcription PCR: First-strand cDNA (cDNA/RNA heteromer) synthesis using customer provided purified total RNA.MBS-6104

Sample Submission Guidelines for gDNA/RNA Extraction Projects

Plasmid Preparation Services

Service TypeDescriptionService
Standard Plasmid PreparationMiniprep Service Include: Transformation into E.coli cells, Culture of Bacteria and Plasmid purification using commercial kits. DNA yield of 2 to 10ugMBS-4001
Midiprep Service Include: Transformation into E.coli cells, Culture of Bacteria and Plasmid purification using commercial kits. DNA yield of 50 to 500ugMBS-4002
Maxiprep Service Include: Transformation into E.coli cells, Culture of Bacteria and Plasmid purification using commercial kits. Minimum DNA yield of 100 to 1000ugMBS-4003
Custom Yield Plasmid PreparationPlasmid Preparation Service – Guaranteed Yield Include: Transformation into E.coli cells, Culture of Bacteria and Plasmid purification using commercial kits. DNA yield as per requestPlease enquire
Other Plasmid Preparation ServiceBacterial stab cultureMBS-4004
Bacterial glycerol stockMBS-4005
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Sample Preparation Guideline