Our team is well-versed in Molecular Applications, in particular PCR amplification, gene expression and copy number quantification by qualitative PCR (qPCR). The offer of our PCR services is now coupled with sample preparation services, allowing you to get the results in the shortest turnaround time, so that you can focus on the data analysis and decide the next move of your research experiments.

Standard PCR

PCR optimization usually takes long time and huge effort. Among many problems to solve in the optimization process, mis-priming and primer dimer that frequently occur in a PCR mixture at room temperature result in poor amplification and undesired amplicon production.

The polymerase chain reaction, or PCR, is one of the key techniques in molecular biology, amplifying a single DNA molecule into millions of copies in a short time by a biochemical process. It has a wide range of applications from basic research to disease diagnostics, agrigenomics, and forensics. 1st BASE provides optimization of PCR assay for high reproducibility and accuracy PCR amplification.

PCR Amplification Assay Optimization

Includes

gDNA extraction, primer design, primer synthesis, all PCR reagents and Purification.
Customer to provide template DNA and positive control template

Human

Plant

Animal

Bacteria

Fungi

Other Organism

Service

MBS-2301

MBS-2302

MBS-2303

MBS-2304

MBS-2305

MBS-2306

 

PCR Amplification Screening

Includes

gDNA extraction& PCR reactions

Forward & Reverse Sequencing.

Gel Quantification

Fragment Analysis

RE digestion

Service

MBS-2310

MBS-2311

MBS-2312

MBS-2313

 

Quantitative PCR (qPCR)

Real-time polymerase chain reaction (qPCR) is a laboratory technique based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real time PCR is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety.

Real time PCR

 

Gene expression

Copy number determination

Assay Optimization

Includes total RNA extraction, primer design, primer synthesis and all qPCR reagents. Customer to provide template DNA and positive control template.
Florescent probes (if any) to charge separately.

Service

MBS-2401

MBS-2402

 

 

Gene Expression

Gene Copy Number

 Includes

Screening using existing MBS ID
Includes nucleic acid (DNA/RNA) extraction, RT-PCR (if any) and qPCR run. Price per run.

Technical replicates

Duplicates

Duplicates

Triplicates

Service

MBS-2403

MBS-2404

MBS-2405

 

Step 1: Download Order Form

Download and complete 1st BASE MBS Order Form (under Downloads tab) with your order requirements.

Step 2: Email the form to our email address below:

Singapore
Email: mbs@axilscientific.com
Tel: +65 6775 7318

Malaysia / International
Email: mbs@apicalscientific.com
Tel: +603 8943 3252

For customers who ship or courier us the orders directly, please indicate your airway bill number or courier tracking number in your email to ensure speedy processing of your orders.

Order Forms Singapore Malaysia/International
Sample Preparation Services
PCR Services
Cloning and Synthetic Biology
Genotyping Services
DNA Barcoding Services
Site-directed Mutagenesis (SDM)