Our team is well-versed in Molecular Applications, in particular PCR amplification, gene expression and copy number quantification by qualitative PCR (qPCR). The offer of our PCR services is now coupled with sample preparation services, allowing you to get the results in the shortest turnaround time, so that you can focus on the data analysis and decide the next move of your research experiments.
PCR optimization usually takes long time and huge effort. Among many problems to solve in the optimization process, mis-priming and primer dimer that frequently occur in a PCR mixture at room temperature result in poor amplification and undesired amplicon production.
The polymerase chain reaction, or PCR, is one of the key techniques in molecular biology, amplifying a single DNA molecule into millions of copies in a short time by a biochemical process. It has a wide range of applications from basic research to disease diagnostics, agrigenomics, and forensics. 1st BASE provides optimization of PCR assay for high reproducibility and accuracy PCR amplification.
PCR Amplification Assay Optimization
gDNA extraction, primer design, primer synthesis, all PCR reagents and Purification.
PCR Amplification Screening
gDNA extraction& PCR reactions
Forward & Reverse Sequencing.
Quantitative PCR (qPCR)
Real-time polymerase chain reaction (qPCR) is a laboratory technique based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real time PCR is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety.
Real time PCR
Copy number determination
Includes total RNA extraction, primer design, primer synthesis and all qPCR reagents. Customer to provide template DNA and positive control template.
Gene Copy Number
Screening using existing MBS ID