PCR optimization usually takes long time and huge effort. Among many problems to solve in the optimization process, mis-priming and primer dimer that frequently occur in a PCR mixture at room temperature result in poor amplification and undesired amplicon production.

The polymerase chain reaction, or PCR, is one of the key techniques in molecular biology, amplifying a single DNA molecule into millions of copies in a short time by a biochemical process. It has a wide range of applications from basic research to disease diagnostics, agrigenomics, and forensics. 1st BASE provides optimization of PCR assay for high reproducibility and accuracy PCR amplification.

Real-time polymerase chain reaction (qPCR) is a laboratory technique based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real time PCR is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety.