Leveraging our expertise in DNA sequencing, we offer DNA barcoding/identification services for pure isolates of bacterial, fungal, plant and animal samples. DNA barcoding/identification services for mixed isolates of bacterial and fungal samples are also available to sequence their cloned product.

Molecular methods will be employed to give more accurate and reproducible results and identifying the samples down to the genus level.

Appropriate controls are used at all levels to ensure the results are consistent and of the highest quality. All reports include the assembled sequences of your samples and the alignment of the top 10 hits to GenBank databases. 

Features of 1st BASE DNA Barcoding Services:

  • Barcoding and identification services for isolated pure samples
  • Accurate and reproducible results
  • Fast turnaround time
  • Bidirectional sequencing prevents ambiguity in sequence
  • Sequences are matched against a standard database, or a database of choice
  • Comprehensive reports include sequences of sample, and the top 10 Hits of the BLAST results
  • Neighbour Joining (unrooted tree)
  • Additional analyses e.g. Phylogenetic analysis between samples is available at separate charges
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Our DNA barcoding services is now coupled with sample preparation services, allowing you to get the results in the shortest turnaround time, so that you can focus on the data analysis and decide the next move of your research experiments.

Bacteria

Description

Service

Bacterial DNA Barcoding (pure isolates), full-length of 16s rRNA (~1400bp).

  • Extraction of gDNA
  • PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database)

MBS-5002

Bacterial DNA Barcoding from mixed isolates, full length of 16s rRNA (~ 1400 bp).

  • Extraction of gDNA, PCR Amplification and Purification
  • Cloning and 8 positive clones send for Bidirectional Sequencing
  • Data analysis (Alignment & BLAST to show the top 10 matches).

MBS-5102

Eukaryote

Description

Service

Eukaryote DNA Barcoding, 18s rRNA (~1800bp)

  • Extraction of gDNA
  • PCR amplification and Purification
  • Primer walking of Cloned DNA
  • Data analysis (BLAST to show the top 10 matches from database)

MBS-5006

 

Fish

Description

Service

Fish DNA Barcoding, COI (~700bp)

  • Extraction of gDNA
  • PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database).

MBS-5009

 

Fungi

Description

Service

Fungal DNA Barcoding (pure isolates), ITS (~700bp)

  • Extraction of gDNA
  • PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database).

MBS-5004

Fungal DNA Barcoding from mixed isolates, ITS (~ 700 bp).

  • Extraction of gDNA, PCR Amplification and Purification
  • Cloning and 8 positive clones send for Forward Single Pass Sequencing
  • Data analysis (Alignment & BLAST to show the top 10 matches from database).

MBS-5104

 

Insect

Description

Service

Insect DNA Barcoding, COI (~700bp)

  • Extraction of gDNA
  • PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database).

MBS-5010

 

Plant

Description

Service

Plant DNA Barcoding, rbcl (~600bp) and/ or matK (~850bp)

  • Extraction of gDNA
  • PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database)

MBS-5003

 

Custom DNA Barcoding

Description

Service

Custom DNA Barcoding: primer sequence provided by customer with minimum of 5 samples (MOQ=5) per order. Price per sample.

  • Extraction of gDNA
  • PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database)

MBS-5007

 

 

Step 1: Download Order Form

Download and complete 1st BASE MBS Order Form (under Downloads tab) with your order requirements.

Step 2: Email the form to our email address below:

Singapore
Email: mbs@axilscientific.com
Tel: +65 6775 7318

Malaysia / International
Email: mbs@apicalscientific.com
Tel: +603 8943 3252

For customers who ship or courier us the orders directly, please indicate your airway bill number or courier tracking number in your email to ensure speedy processing of your orders.


Click below for Guides on Preparations and Requirements

Order Forms Singapore Malaysia/International
Sample Preparation Services
PCR Services
Cloning and Synthetic Biology
Genotyping Services
DNA Barcoding Services
Site-directed Mutagenesis (SDM)