Complete Plasmid Validation with Fast, Accurate Long-Read Sequencing
Get your complete plasmid sequence with Whole Plasmid Sequencing by 1st BASE - a fast and reliable solution powered by advanced long-read sequencing technology.
Unlike traditional Sanger sequencing that only verifies selected regions, Whole Plasmid Sequencing provides full plasmid coverage without primer design, delivering comprehensive sequence validation within 1–2 business days.
From cloning confirmation to vector development and quality control, our service provides the complete data package you need to confidently move your research forward.
Why Choose Whole Plasmid Sequencing by 1st BASE?
Complete Plasmid Coverage
Obtain the full sequence of your plasmid with end-to-end coverage, including challenging regions that may be missed by conventional sequencing approaches.
Primer-Free Sequencing
No primer design or optimization is required. Simply submit your plasmid DNA and receive complete sequencing results.
Fast Turnaround Time
Receive comprehensive sequencing data within 1–2 business days, helping you accelerate your research workflow.
Comprehensive Data Package
Every sequencing run includes:
✓ Annotated GenBank file
✓ Plasmid map (PDF)
✓ Consensus FASTA sequence
✓ Raw sequencing reads (FASTQ)
✓ QC report with contamination scoring
Whole Plasmid Sequencing vs Traditional Sanger Sequencing
| Feature | Whole Plasmid Sequencing | Traditional Sanger Sequencing |
|---|---|---|
| Coverage | Complete plasmid sequence | Partial sequence verification |
| Primer Requirement | Primer-free | Requires primer design |
| Complex Regions | Better resolution of repetitive and GC-rich regions | May require multiple primers |
| Variant Detection | Detects SNPs, indels and structural variations | Limited detection capability |
| Application | Full plasmid validation and characterization | Targeted insert confirmation |
Applications of Whole Plasmid Sequencing
Whole Plasmid Sequencing is ideal for:
Vector Quality Control
Confirm plasmid identity, sequence integrity, and construct accuracy before downstream experiments.
AAV & ITR Validation
Validate complex viral vectors and inverted terminal repeat (ITR) regions.
Pre-Release Plasmid Verification
Ensure final plasmid constructs meet quality requirements before distribution or further development.
Library Characterization
Characterize plasmid libraries and confirm sequence diversity.
Simple Workflow
1. Submit Your Sample
Provide plasmid DNA according to submission requirements.
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2. Sample Collection
Our team arranges sample pickup.
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3. Long-Read Sequencing
Your plasmid undergoes high-resolution sequencing using advanced long-read technology.
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4. Assembly & Annotation
Raw sequencing data is assembled, annotated, and quality assessed.
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5. Result Delivery
Receive your complete sequencing package with QC documentation.
Sample Submission Requirements
Plasmid DNA Requirements
| Plasmid Size | Concentration | Volume |
|---|---|---|
| ≤10 kb | ≥20 ng/µl | ≥20 µl |
| 10–20 kb | ≥30 ng/µl | ≥20 µl |
| ≥20 kb | ≥40 ng/µl | ≥20 µl |
Purity Requirements
- OD260/280: 1.8–2.0
- OD260/230: ≥2.0
Sample format:
Plasmid DNA in 1.5 ml tube