Sample Preparation Services

At 1st BASE, we have the in-house expertise in processing samples for downstream molecular biology applications routinely. Researchers may depend on us to process their samples for their processes without fuss. 

Services include:

  • Genomic DNA Extraction from environmental samples (e.g. water, sludge, fecal, soil and etc.), animal tissues, animal blood/ saliva, insect, fungi, bacteria, plant leaves, plant fruits and plant seeds.
  • Total RNA Extraction from environmental samples (e.g. soil), animal tissues, animal blood, insect, fungi, bacteria, plant leaves, plant fruits and plant seeds
  • RNA analysis by Labchip/ Biochip
  • DNA Digestion by RE
  • Plasmid Preparation for various yield from 2ug to 1000ug.
  • Reverse Transcription PCR (RT-PCR) from total RNA to cDNA

The process of sample preparation is tedious and the amount of samples are typically limited for research. To maximise success for each sample preparation service, we advise our customers on how to collect and submit the samples within the acceptable terms and conditions. Such information is also available in each of the respective order forms. Minimum setup fee will apply when sample preparation service fails due to bad quality of sample that did not meet the prescribed terms and conditions.

 

Specifications

 Product No. Description
MBS-6001 DNA Preparation
Standard Genomic DNA (gDNA) up to 100mg of starting material. Price per sample.
MBS-6002 DNA Analysis & Preparation
DNA digestion using Restriction Enzyme (RE), up to 2 RE. Price per sample.
MBS-6008 DNA Preparation
Purification of Genomic DNA (gDNA) to remove impurities that inhibits PCR. Price per purification.
MBS-6103 RNA Preparation
Standard Total RNA Extraction up to 100mg of starting material. Price for per sample.
MBS-6100 RNA LabChip Analysis
Total RNA (25 to 500 ng/uL), Eukaryotic, 18S and 28S, size 25 - 6000 bp. Price per chip (up to 12 samples).
MBS-6101 RNA LabChip Analysis
Total RNA (25 to 500 ng/uL), Prokaryotic, 16S and 23S, size 25 - 6000 bp. Price per chip (up to 12 samples).
MBS-6102 RNA LabChip Analysis
Total RNA (25 to 500 ng/uL), Eukaryotic (Plant), 18S and 25S, size 25 - 6000 bp. Price per chip (up to 12 samples).
MBS-4001

Custom Bacterial Plasmid Miniprep Service
Include: Transformation into E.coli cells, Culture of Bacteria and Plasmid purification using commercial kits.
Final Product: Minimum DNA yield of 2 to 10ug per experiment

MBS-4002 Custom Bacterial Plasmid Midiprep Service 
Include: Transformation into E.coli cells, Culture of Bacteria and Plasmid purification using commercial kits.
Final Product: Minimum DNA yield of 50 to 500ug per experiment
MBS-4003

Custom Bacterial Plasmid Maxiprep Service
Include: Transformation into E.coli cells, Culture of Bacteria and Plasmid purification using commercial kits.
Final Product: Minimum DNA yield of 100 to 1000ug per experiment

MBS-4004 Plasmid Preparation Service
Bacterial stab culture, price per tube. Ship in blue ice condition.
MBS-4005 Plasmid Preparation Service
Bacterial glycerol stock, price per tube. Ship in dry ice condition.
MBS-6104 RNA Preparation
Reverse Transcription PCR: First-strand cDNA (cDNA/RNA heteromer) synthesis using customer provided purified total RNA.