Sample Preparation Services
At 1st BASE, we have the in-house expertise in processing samples for downstream molecular biology applications routinely. Researchers may depend on us to process their samples for their processes without fuss.
- Genomic DNA Extraction from environmental samples (e.g. water, sludge, fecal, soil and etc.), animal tissues, animal blood/ saliva, insect, fungi, bacteria, plant leaves, plant fruits and plant seeds.
- Total RNA Extraction from environmental samples (e.g. soil), animal tissues, animal blood, insect, fungi, bacteria, plant leaves, plant fruits and plant seeds
- RNA analysis by Labchip/ Biochip
- DNA Digestion by RE
- Plasmid Preparation for various yield from 2ug to 1000ug.
- Reverse Transcription PCR (RT-PCR) from total RNA to cDNA
The process of sample preparation is tedious and the amount of samples are typically limited for research. To maximise success for each sample preparation service, we advise our customers on how to collect and submit the samples within the acceptable terms and conditions. Such information is also available in each of the respective order forms. Minimum setup fee will apply when sample preparation service fails due to bad quality of sample that did not meet the prescribed terms and conditions.
Standard Genomic DNA (gDNA) up to 100mg of starting material. Price per sample.
|MBS-6002||DNA Analysis & Preparation
DNA digestion using Restriction Enzyme (RE), up to 2 RE. Price per sample.
Purification of Genomic DNA (gDNA) to remove impurities that inhibits PCR. Price per purification.
Standard Total RNA Extraction up to 100mg of starting material. Price for per sample.
|MBS-6100||RNA LabChip Analysis
Total RNA (25 to 500 ng/uL), Eukaryotic, 18S and 28S, size 25 - 6000 bp. Price per chip (up to 12 samples).
|MBS-6101||RNA LabChip Analysis
Total RNA (25 to 500 ng/uL), Prokaryotic, 16S and 23S, size 25 - 6000 bp. Price per chip (up to 12 samples).
|MBS-6102||RNA LabChip Analysis
Total RNA (25 to 500 ng/uL), Eukaryotic (Plant), 18S and 25S, size 25 - 6000 bp. Price per chip (up to 12 samples).
Custom Bacterial Plasmid Miniprep Service
|MBS-4002||Custom Bacterial Plasmid Midiprep Service
Include: Transformation into E.coli cells, Culture of Bacteria and Plasmid purification using commercial kits.
Final Product: Minimum DNA yield of 50 to 500ug per experiment
Custom Bacterial Plasmid Maxiprep Service
|MBS-4004||Plasmid Preparation Service
Bacterial stab culture, price per tube. Ship in blue ice condition.
|MBS-4005||Plasmid Preparation Service
Bacterial glycerol stock, price per tube. Ship in dry ice condition.
Reverse Transcription PCR: First-strand cDNA (cDNA/RNA heteromer) synthesis using customer provided purified total RNA.