Simplify Digestion Protocol of Proteins for LC-MS/Proteomics with
ProteoBind On-column Proteolytic Digestion Kit
With Technology Licensed from A*STAR, Singapore
1st BASE ProteoBind On-column Proteolytic Digestion Kit is a comprehensive solution for efficient protein digestion in various samples, including cell lysates, protein extracts, and purified proteins. This kit features a streamlined workflow and optimized reagents, ensuring complete protein digestion with a high recovery rate of peptides. The protocol is designed for rapid, highly reproducible, and versatile protein digestion, providing results with high confidence. The simple spin procedure allows for simultaneous processing of multiple samples, yielding protein digests ready for mass spectrometry (MS) analysis in just 70 minutes.
- Consistency: Standardised protocols that minimizes variability and ensure reproducibility, leading to consistent results across different experiments and users.
- Convenience: All necessary reagents and buffers are provided in a ready-to-use format, eliminating the need for time-consuming preparation steps.
- Efficiency: Streamlined workflow and optimized reagents guarantee complete protein digestion with high recovery rates of peptides, saving time and resources.
- Versatility: Suitable for a variety of protein samples, including cell lysates, protein extracts, and purified proteins, making it adaptable to different research needs.
- Rapid Results: Offers a rapid digestion protocol, delivering protein digests ready for mass spectrometry (MS) analysis in just 70 minutes, enhancing productivity.
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Application Data
Lane 1: ExactPro Prestained Protein Ladder
Lane 2: BSA Stock
Lane 3: ProteoBind Digested - BSA
Lane 4: Brand P Digested - BSA
Lane 5: FBS Stock Diluted 20X
Lane 6: ProteoBind Digested - FBS
Lane 7: Brand P Digested - FBS
Lane 8: ProteoBind Digested NFW
Lane 9: Brand P Digested NFW
Lane 10: SDS-PAGE NFW Negative Control
Lane 11: Mark12 Unstained Standard
The successful digestion of BSA and FS by both ProteoBind On-Column Proteolytic Digestion Kit and Brand P methods was indicated by the absence of the 66kDa target protein in the digested samples and the presence of 10kDa peptides.
The LCMS data indicated that all samples were successfully digested. Based on the intensity, ProteoBind-digested BSA and FBS exhibited higher intensity, suggesting a potentially higher abundance.
Both ProteoBind and Brand P were loaded with 45.56 µg to measure the concentration of digested protein. The concentrations were measured using the BCA Protein Assay (Polynomial 3). In the graph, ProteoBind shows a higher concentration of digested protein compared to Brand P, and this concentration remains at a consistent level throughout the assay.
The concentration of digested protein for both ProteoBind and Brand P, with an amount loaded for digestion of 50.60 µg. The diagram shows that the percentage of protein lost is significantly lower for ProteoBind compared to Brand P.
| Product Description | Reactions | Product No. | Product Manual |
| ProteoBind On-column Proteolytic Digestion Kit | 10 | BIO-5300-10 |  |
| 50 | BIO-5300-50 |
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