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Taq II Polymerase and PCR Master Mix

Superior Performance on Fidelity, Stability and Specificity.

Taq II polymerase is an enhanced Taq DNA polymerase optimised for routine PCR applications. The enzyme provides a higher sensitivity, yields and longer PCR amplification as compared to conventional Taq DNA polymerase. It is supplied with optimised Taq II Polymerase 10X buffer and 25mM MgCl2, minimising the need for optimisation.

Highlights

  • Robust amplification
  • Higher sensitivity as compared to conventional Taq
  • Amplification of targets up to 6kb from genomic DNA
  • High efficiency and PCR yields
  • Fast – developed to work under fast thermal cycling conditions

Polymerase for everyday applications

  • DNA sequencing
  • DNA labelling
  • PCR for cloning
  • PCR ampli­fication of DNA fragments up to 6kb
  • Routine PCR

Specifications

Fidelity 1X
Amplification length Up to 6 kb
Overhang Sticky ends/ 3'-A
Polymerase Taq II DNA polymerase
Amplification speed 30s-60s/kb

Robust and efficient amplification

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Better Sensitivity

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Faster processivity

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REDiant II DNA Polymerase & Master Mix

Formulated with Density Reagents and Inert Tracking Dyes to Enable Easy Visual Control

REDiant II Polymerase and PCR Mastermix uses the enhanced Taq II polymerase and are formulated with density reagents and inert tracking dyes to enable easy visual control during PCR set-up and direct loading of the reaction product into the gel. REDiant II PCR Master Mix is a premixed, ready to use solution which contains Taq DNA polymerase, dNTPs, MgCl2 and reaction buffer for amplification of DNA templates by PCR. This pre-mixed formulation saves time and reduces contamination by reducing the number of pipetting steps required for usual PCR set up. The mix is optimised for efficient and reproducible PCR. It also includes a red, inert tracking dye which migrates at the same rate as 1kb DNA fragment in a 1% agarose gel.

Highlights

  • Direct loading of PCR products onto agarose gel without addition of gel loading buffer
  • Visualizes the addition of polymerase into the PCR reaction mix and ensure complete mixing
  • Acts as tracking dye in gel electrophoresis
  • Red inert dye does not inhibit PCR

Polymerase for everyday applications

  • DNA sequencing
  • DNA labelling
  • PCR for cloning
  • PCR ampli­fication of DNA fragments up to 6kb
  • Routine PCR

Specifications

Fidelity 1X
Amplification length Up to 6 kb
Overhang Sticky ends/ 3'-A
Polymerase Taq II DNA polymerase
Reaction speed 30s-60s/kb

Robust and efficient amplification

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Higher Sensitivity

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Faster Processivity

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Convenient & Ease of Handling

REDiant II contains red tracking dye to save time and prevent cross contamination between samples. The red tracking dye does not interfere with the PCR and serves as a loading dye for gel electrophoresis to monitor the progress of a running gel. The dye migrates at similar rate to a 500bp-800bp DNA fragment in a 1% agarose gel.

exTEN II PCR Master Mix

exTENsively Amplifies Up to 10kb

exTEN II is an unique premixed blend of Taq DNA polymerase, a proof-reading enzyme, dNTPs, MgCl2 and reaction buffer for amplification of DNA templates by PCR With the addition of a 3’ 5’ exonuclease (proof-reading) ability, the amplification efficiency is enhanced through a lower rate misincorporated nucleotides compared to just Taq DNA Polymerase alone.

exTEN II PCR Master Mix produces higher yields and amplifies longer fragments up to 10kb. Most of the amplified DNA fragments have 3’A overhang, while a small percentage are blunt-end. This premix formulation saves times and reduces contamination by reducing the number of pipetting steps for PCR setup. exTEN II PCR Master Mix consists of a density reagent and 2 tracking dyes which migrates at the same rate as a 4000bp and 50bp DNA fragment in a 1% agarose gel.

Highlights

  • Superior yield and robust amplification of DNA fragments up to 10kb
  • Greater sensitivity achieved with low concentration template DNA
  • Minimum pipetting steps required as it is formulated for easy preparation and direct loading which also reduces contamination & errors

 

Specification

Fidelity 1X
Amplification length Up to 10 kb
Overhang Mix of sticky & blunt ends
Polymerase Taq II DNA polymerase & proof-reading enzyme
Amplification speed 30s-60s/kb

Superior Yield & Robust Amplification of DNA Fragments Up to 10kb

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Better Sensitivity Achieved even in Low Concentration of DNA Template

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Superior Performance

 

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Convenient & Ease of Handling

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Product Product Description Product No. Product Manual Safety Data Sheet
Taq II Taq II DNA Polymerase (recombinant), 500U BIO-5111-500U
Taq II DNA Polymerase (recombinant), 2500U BIO-5111-2500U
Taq II PCR Master Mix, 200 reactions BIO-5181-200
Taq II PCR Master Mix, 1000 reactions BIO-5181-1000
REDiant II REDiant Taq II DNA Polymerase (recombinant), 500U BIO-5116-500U
REDiant Taq II DNA Polymerase (recombinant), 2500U BIO-5116-2500U
REDiant II PCR Master Mix (2X), 200 reactions BIO-5187-200
REDiant II PCR Master Mix (2X), 1000 reactions BIO-5187-1000
exTEN II exTEN II PCR Master Mix (2X), 200 reactions BIO-5188-200
exTEN II PCR Master Mix (2X), 1000 reactions BIO-5188-1000
Contact Us for Orders or Enquiries

Select Your Country

Sample Request Form

Our new enhanced PCR reagents are available in 50U sample sizes. Complete the form below and get a free sample delivered to your lab.

Master mix is convenient and easy to use but may not be more suited for routine and uncomplicated PCRs such as genotyping.

REDiant II PCR Master Mix (2X)

Hummingbird Bioscience Pte Ltd
Singapore

Comparing REDiant II and its predecessor, REDiant, REDiant II produced much more obvious bands for zebrafish caudal fin samples. Note: Exact same samples and primer combinations with the same PCR steps were applied for comparison.

BIO-5187-20 – REDiant II PCR Master Mix (2X), 20 reactions

Singapore Nuclear Research and Safety Initiative (SNRSI), National University of Singapore
Singapore

Efficient to get the most desired results

BIO-5187 – REDiant II PCR Master Mix (2X)

SIFBI
Singapore

exTEN II works great for our experiments, definitely considering the product moving forward.

BIO-5188 – exTEN II PCR Master Mix (2X)

ArrowBiome
Singapore

The high fidelity version (exTEN II) worked well. Extracted my gene of interest and the sequencing results were good. Happy about it.

BIO-5188 – exTEN II PCR Master Mix (2X)

Singapore-MIT Alliance for research and technology
Singapore

Fairly easy to use, great without needing loading dye.

BIO-5187-20 – REDiant II PCR Master Mix (2X), 20 reactions

ASRL
Singapore

Product is good and has comparable performance with other enzymes in the market.

BIO-5116 – REDiant II Taq DNA Polymerase (recombinant) – MgCl₂ separate

BIO-5188 – exTEN II PCR Master Mix (2X)

Wilmar International
Singapore

Reagent mix is efficient and gives a clean band during colony pcr screening step

BIO-5187-20 – REDiant II PCR Master Mix (2X)

BTI
Singapore

REDiant Gen II produces bands with slightly higher intensity than exTEN Gen II.

BIO-5187 – REDiant II PCR Master Mix (2X)

BIO-5188 – exTEN II PCR Master Mix (2X)

Wilmar International
Singapore

It seem to be work well compared to high fidelity mix which we bought for pcr product cloning.

BIO-5116 – REDiant II Taq DNA Polymerase (recombinant) – MgCl₂ separate

BTI
Singapore

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