Taq II Polymerase and PCR Master Mix

Superior Performance on Fidelity, Stability and Specificity.

Taq II polymerase is an enhanced Taq DNA polymerase optimised for routine PCR applications. The enzyme provides a higher sensitivity, yields and longer PCR amplification as compared to conventional Taq DNA polymerase. It is supplied with optimised Taq II Polymerase 10X buffer and 25mM MgCl₂, minimising the need for optimisation.

Highlights

• Robust amplification
• Higher sensitivity as compared to conventional Taq
• Amplification of targets up to 6kb from genomic DNA
• High efficiency and PCR yields
• Fast – developed to work under fast thermal cycling conditions

Polymerase for everyday applications

• DNA sequencing
• DNA labelling
• PCR for cloning
• PCR ampli­fication of DNA fragments up to 6kb
• Routine PCR

Specifications

Robust and efficient amplification

Better Sensitivity

Faster processivity

REDiant II DNA Polymerase & Master Mix

Formulated with Density Reagents and Inert Tracking Dyes to Enable Easy Visual Control

REDiant II Polymerase and PCR Mastermix uses the enhanced Taq II polymerase and are formulated with density reagents and inert tracking dyes to enable easy visual control during PCR set-up and direct loading of the reaction product into the gel. REDiant II PCR Master Mix is a premixed, ready to use solution which contains Taq DNA polymerase, dNTPs, MgCl2 and reaction buffer for amplification of DNA templates by PCR. This pre-mixed formulation saves time and reduces contamination by reducing the number of pipetting steps required for usual PCR set up. The mix is optimised for efficient and reproducible PCR. It also includes a red, inert tracking dye which migrates at the same rate as 1kb DNA fragment in a 1% agarose gel.

Highlights

• Direct loading of PCR products onto agarose gel without addition of gel loading buffer
• Visualizes the addition of polymerase into the PCR reaction mix and ensure complete mixing
• Acts as tracking dye in gel electrophoresis
• Red inert dye does not inhibit PCR

Polymerase for everyday applications

• DNA sequencing
• DNA labelling
• PCR for cloning
• PCR ampli­fication of DNA fragments up to 6kb
• Routine PCR

Specifications

Robust and efficient amplification

Higher Sensitivity

Faster Processivity

Convenient & Ease of Handling

REDiant II contains red tracking dye to save time and prevent cross contamination between samples. The red tracking dye does not interfere with the PCR and serves as a loading dye for gel electrophoresis to monitor the progress of a running gel. The dye migrates at similar rate to a 500bp-800bp DNA fragment in a 1% agarose gel.

exTEN II PCR Master Mix

exTENsively Amplifies Up to 10kb

exTEN II is an unique premixed blend of Taq DNA polymerase, a proof-reading enzyme, dNTPs, MgCl2 and reaction buffer for amplification of DNA templates by PCR With the addition of a 3’ 5’ exonuclease (proof-reading) ability, the amplification efficiency is enhanced through a lower rate misincorporated nucleotides compared to just Taq DNA Polymerase alone.

exTEN II PCR Master Mix produces higher yields and amplifies longer fragments up to 10kb. Most of the amplified DNA fragments have 3’A overhang, while a small percentage are blunt-end. This premix formulation saves times and reduces contamination by reducing the number of pipetting steps for PCR setup. exTEN II PCR Master Mix consists of a density reagent and 2 tracking dyes which migrates at the same rate as a 4000bp and 50bp DNA fragment in a 1% agarose gel.

Highlights

• Superior yield and robust amplification of DNA fragments up to 10kb
• Greater sensitivity achieved with low concentration template DNA
• Minimum pipetting steps required as it is formulated for easy preparation and direct loading which also reduces contamination & errors

Specification

Superior Yield & Robust Amplification of DNA Fragments Up to 10kb

Better Sensitivity Achieved even in Low Concentration of DNA Template

Superior Performance

Convenient & Ease of Handling

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