AmpliPLUS HiFi PCR Master Mix (2X) contains a modified DNA polymerase, Mg2+, dNTPs, and PCR stabilisers and enhancers, all optimised in a reaction buffer for robust and versatile high-fidelity PCR of long fragments. This unique polymerase excels at amplifying regions with high GC content at a speed of 20secs/kb.

AmpliPLUS is approximately 100 times higher than conventional Taq DNA polymerase, making it an ideal alternative for applications where higher accuracy is needed.

With rapid amplification capabilities, optimised efficiency for challenging templates, and robust performance across diverse sample types, AmpliPLUS emerges as a versatile and reliable solution.

Interested to try it out?

Fig 1. Fidelity comparison across commercially available products.

AmpliPLUS ensures the precise replication of genetic material, boasting an accuracy level approximately 100 times greater than Taq DNA polymerase. This makes it a preferred choice for applications requiring heightened accuracy without a substantial rise in the cost per reaction.

PCR efficiency can be affected by DNA templates with high GC content (>65%) due to the tendency of these templates to fold into complex secondary structures. AmpliPLUS is specifically designed to excel in amplifying regions characterised by high GC content.

Fig 2. Nucleic acids extracted from human cell pellet with high GC regions:

1. 1. Amplicon: 752bp, GC content: 64.0%
   2. Amplicon: 771bp, GC content: 69.5%
   3. Amplicon: 714bp, GC content: 75.9%

AmpliPLUS offers robust amplification, providing high yields for different sample types and templates of varying length. It is compatible with DNA templates from various sample types, including human cells, yeast, leaves, or stool samples.

Fig 3. Nucleic acids were extracted from various sample types of different length using AmpliPLUS and other competing brands:

1. 1. Yeast pellet, ~500bp
   2. Leaf, ~800bp
   3. Mouse stool, ~1400bp
   4. Human cell pellet. ~3500bp

The combination of fast amplification and high fidelity in a PCR master mix is essential for achieving reliable and timely results. AmpliPLUS is capable of amplifying DNA template with an extension speed of 20 seconds/kb or less.

Fig 4. Different DNA templates were amplified using AmpliPLUS with different extension speed:

1. 1. 5 seconds/kb
   2. 10 seconds/kb
   3. 15 seconds/kb
   4. 20 seconds/kb

Fig 5a. Amplification of 16S, Mouse stool, on different amount of DNA templates starting from 10.0ng to 0.1ng in 50ul reaction volume.

• • Lane 1 - 1kb Marker
  • Lane 2 - 0.1ng input 16S in Mouse Stool ~1400bp
  • Lane 3 - 1.0ng input 16S in Mouse Stool ~1400bp
  • Lane 4 - 2.5ng input 16S in Mouse Stool ~1400bp
  • Lane 5 -  10.0ng input 16S in Mouse Stool ~1400bp

Fig 5b. Nucleic acid extracted from human gDNA and amplified with CECR6 (high GC target) with varying amount (in ng) of input nucleic acid in 20ul reaction volume.

• • Lane 1 - 100bp Marker
  • Lane 2 - 0.1ng input DNA
  • Lane 3 - 1.0ng input DNA
  • Lane 4 - 2.5ng input DNA
  • Lane 5 -  10.0ng input DNA
  • Lane 6 - Negative Control