Fragment Analysis Services Order Preparation and Submission

Standard Fragment Analysis
Human Cell Line Authentication


 

 

Standard Fragment Analysis

Submit your samples using strip capped 96-well plate, 8-PCR strips, or 1.5mL micro tubes. Kindly wrap them with aluminium foil as some fluorescent dyes may easily degrade without light protection.

Tips on packing your samples in a 96-well plate

  1. Please place your samples properly into strip-capped well plate as shown below. Adhesive seals or sealing mats will cause mix-up of wells during transportation.
  2. To minimize the physical damage, it is best to use out-skirted well plate with parafilm.
  3. Ensure equal volume; and seal tightly with 8-PCR strip cap to avoid any potential damages in transit such as evaporation or contamination of samples during shipping.
  4. Bad sealing may lead cross-contamination and unsatisfactory results!

 

 

 

Human Cell Line Authentication

Sample Type

Sample Requirements

Purified Genomic DNA

>10ng/µL in 10µL

Cell Pellet*

  1. Grow cell in T25 Flask (or equivalent) until 70% confluent.
  2. Harvest cells and make aliquots into 2 to 3 tubes
  3. Wash with 1x PBS.
  4. Store and ship cell pellets in dry ice.

*gDNA extraction charge applies

FTA Card

  1. Prepare samples one at a time at an optimal target cell density of 1 x 106 cells/mL.
    a. For attached cells: After trypsinization, wash cells with 1X PBS. Re-suspend the cells in a small volume of PBS, count the cells and dilute the sample to 1 x 106 cells/mL.
    b. For suspension cells: Harvest and count cells.
    Note: If cell density is less than 1 x 106 cells/mL, must re-centrifuge and re-suspend in PBS to get a final concentration of 1 x 106 cells/mL. If cell density is greater than 1.7 x 106 cells/mL, then dilute the sample in PBS to 1 x 106 cells/mL.
     
  2. Clean the work surface in a cell culture hood, open sample storage card with gloved hands.
     
  3. Label the sample storage card. Spot 40 µL of cell suspension from step 1 in the centre of each circle of the sample storage card, total 2 - 4 circles per sample.
     
  4. Air dry sample storage card at room temperature.
     
  5. Close sample storage card and put it inside the collection bag (optional with desiccant pack). Ship at room temperature.