Sample Submission Guidelines for gDNA/RNA extraction projects
Sample Type/OriginFor gDNA extractionFor RNA extraction
Soil/Sludge
  • Weight: 1g
  • Storage in -80°C freezer
  • 3 replicates per sample to be sent
N/A
Water
  • Volume: 1L
  • Storage in the dark in 4°C fridge
  • Non leaking bottles or containers
N/A
Bacteria Pellet form (30ml of overnight culture)

Storage in -80°C freezer

3 replicates per sample to be sent

  • Pellet form (30ml of overnight culture)
  • Storage in -80°C freezer
  • 3 replicates per sample to be sent
Fungi
  • Pellet form (30ml of overnight culture) or Frozen fungal material
  • Weight: 100mg
  • Storage in -80°C freezer
  • 3 replicates per sample to be sent
  • Filamentous Fungi material
  • Weight: 100mg
  • Snap frozen in liquid nitrogen
  • Store in -80°C freezer
  • 3 replicates per sample to be sent
Plant
  • Young leaves/needles preferred
  • Weight: 100mg wet weight, 20mg if plant samples is lyophilized
  • Fresh plants samples: snap frozen in liquid nitrogen
  • Store in -80°C freezer
  • Lyophilized plant samples: samples should be completely dried within 24hrs of collection and stored at RT
  • 3 replicates per sample to be sent
  • Young leaves/needles preferred
  • Weight: 100mg wet weight
  • Fresh plants samples: snap frozen in liquid nitrogen
  • Store in -80°C freezer
  • 3 replicates per sample to be sent
Tissue samples (Human or Animal)
  • Tissue must be wash with 1X PBS to remove all traces of blood
  • Weight: 30mg
  • Snap Frozen in liquid nitrogen after 1XPBS wash and weighing.
  • Store in -80°C freezer
  • 3 replicates per sample to be sent
Blood (Human)
  • Volume: 1ml in EDTA Tube
  • Fresh sample only
  • Storage at 4°C fridge
  • Blood samples to be submitted immediately after withdrawal
  • Results will not be guaranteed for blood samples that are more than 3 days old
  • Please enquire before collection/ submission
  • Volume: 3ml in Tempus™ Blood RNA Tubes
  • Fresh sample only
  • Storage at 4°C fridge
  • Blood samples to be submitted immediately after withdrawal
  • Results will not be guaranteed for blood samples that are more than 3 days old
  • Please enquire before collection/ submission
Blood (Animal)
  • Volume: 100ul
  • 3 replicates per sample to be sent
  • Volume: 500ul
  • 3 replicates per sample to be sent
Cultured cells
  • Pellet form
  • 5 x 106
  • Storage in -80°C freezer
  • 3 replicates per sample to be sent
  • Pellet form
  • 1 x 107
  • Storage in -80°C freezer
  • 3 replicates per sample to be sent