1st BASE comprehensive range of protein ladders includes Prestained Protein Ladders in both Broad Range (3.5kDa – 245kDa) and Regular Range (9kDa – 180kDa), as well as Western Markers (15 kDa to 200 kDa). Designed to meet the diverse needs of protein research and analysis, these ladders offer unparalleled accuracy and convenience.

ExactPro Broad Range Prestained Protein Ladder

Product Series: BIO-5150

Prestained Protein Ladder contains 13 prestained recombinant proteins that cover a wide range of molecular weights from 3.5kDa – 245kDa. An orange reference band of ~75kDa and a green reference band of ~25kDa provides easy monitoring of protein separation.

The ladder is ready-to-use. No further dilution, addition of a reducing agent or heating is required.


Storage Buffer

20mM Tris-H3PO4 (pH 7.5 @ 25°C) 15% (v/v) Glycerol 2% (w/v) SDS 10mM DTT 3.6mM Urea


Applications

Used in SDS-PAGE and Western blots for approximation of protein sizes, and also to locate a protein of interest for excision from an unstained preparative gel.


Recommendations

    • Thaw ladder at room temperature to dissolve precipitated solids. Do not boil.
    • Mix solution gently to ensure that it is homogeneous.
    • Load ladder into SDS-PAGE gel or Western blot using these volumes (based on gel thickness of 0.75 – 1.0mm).
      • 3µl per loading on 15-well mini-gel
      • 5µl per loading on 10-well mini-gel
      • 1.5 – 2.5µl per loading for general Western transferring
      • Double the loading volume for thicker or larger gel.

ExactPro Regular Range Prestained Protein Ladder

Product Series: BIO-5151

ExactPro Regular Range (9-180 kDa) Prestained Protein Ladder contains 10 prestained recombinant proteins that cover a range of molecular weights from 9 kDa – 180 kDa. Proteins are covalently coupled with a blue chromophore, with 2 enhanced reference bands, 25 kDa and 75 kDA, when separated on SDS-PAGE (Tris-Glycine). Prestained proteins may have different mobilities in various SDS-PAGE buffer system.

The ladder is ready-to-use. No further dilution, addition of a reducing agent or heating is required.


Storage Buffer

20mM Tris-H3PO4 (pH 7.5 @ 25°C) 15% (v/v) Glycerol 2% (w/v) SDS 0.mM DTT 3.6mM Urea


Applications

Used in SDS-PAGE and Western blots for approximation of protein sizes, and also to locate a protein of interest for excision from an unstained preparative gel.


Recommendations

    • Thaw ladder at room temperature to dissolve precipitated solids. Do not boil.
    • Mix solution gently to ensure that it is homogeneous.
    • Load ladder into SDS-PAGE gel or Western blot using these volumes (based on gel thickness of 0.75 – 1.0mm).
      • 3µl per loading on 15-well mini-gel
      • 5µl per loading on 10-well mini-gel
      • 1.5 – 2.5µl per loading for general Western transferring
    • Double the loading volume for thicker or larger gel.

ExactPro Western Marker

Product Series: BIO-5155

ExactPro Western Marker is a ready-to-use mixture containing 10 IgG-binding proteins with molecular weights ranging from 15 kDa to 200 kDa in Tris-Glycine Buffer. ExactPro Western Marker has 2 enhanced, reference bands (at 30 kDa and 80 kDa).

ExactPro Western Marker is compatible with chemiluminescent, fluorescent, chromogenic, and other detection systems.

The ladder is ready-to-use. No further dilution, addition of a reducing agent or heating is required.


Applications

Used in SDS-PAGE and Western blots for approximation of protein sizes, and to locate a protein of interest for excision from an unstained preparative gel. Visualization of 10 IgG-binding proteins on Western blots.


Recommendations

  • Thaw ladder at room temperature to dissolve precipitated solids.
    Do not boil.
  • Mix solution gently to ensure that it is homogeneous.
  • Load ladder into SDS-PAGE gel or Western blot using these volumes (based on gel thickness of 0.75 – 1.0mm).
    • 5 – 2.5 µl per well for 2-step Western blot utilizing primary antibody followed by secondary antibody conjugated with reporter enzymes
    • 5 – 5 µl per well for 1-step Western blot utilizing primary antibody conjugated with reporter enzymes.
  • Double the loading volume for thicker or larger gel.

ProductExactPro Prestained Protein Ladder,
Broad Range

ExactPro Prestained Protein Ladder, Regular Range New!

ExactPro Western Marker New!
Range3.5kDa – 245kDa9kDA – 180kDa15 kDa – 200 kDa (western bands)

10 kDa – 70 kDa (pre-stained bands)

No. of Bands131010 (Film)

4 (Membrane)

ColorsTri-colorOne colorTri-color
Stained TypeBlue, Red, GreenBlueBlue, Pink, Green
ApplicationsSDS-PAGE

Western blots

SDS-PAGE

Western blots

SDS-PAGE

Western blots

Storage-20oC25°C (2 weeks)

4°C (3 months)

-20°C (long term storage)

    4°C(3 months)

-20°C (24 months)

Pack Size1 x 250ul

1 x 500ul

2 x 250ul1 x 250ul
Product No.BIO-5150-250ul

BIO-5150-500ul

BIO-5151-500ulBIO-5151-250ul

Interested to try it out?

Description Product No. Protocol MSDS
ExactPro Broad Range (5-245kDa) Prestained Protein Ladder BIO-5150-250ul
BIO-5150-500ul

ExactPro Regular Range (9-180 kDa) Prestained Protein Ladder

BIO-5151-25ul    
BIO-5151-500ul    

ExactPro Western Marker

BIO-5155-25ul    
BIO-5155-250ul    
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