Prestained Protein Ladder contains 13 prestained recombinant proteins that cover a wide range of molecular weights from 3.5kDa – 245kDa. An orange reference band of ~75kDa and a green reference band of ~25kDa provides easy monitoring of protein separation.

The ladder is ready-to-use. No further dilution, addition of a reducing agent or heating is required.

Storage Buffer

20mM Tris-H3PO4 (pH 7.5 @ 25°C) 15% (v/v) Glycerol 2% (w/v) SDS 10mM DTT 3.6mM Urea

Applications

Used in SDS-PAGE and Western blots for approximation of protein sizes, and also to locate a protein of interest for excision from an unstained preparative gel.

Recommendations

• • Thaw ladder at room temperature to dissolve precipitated solids. Do not boil.
  • Mix solution gently to ensure that it is homogeneous.
  • Load ladder into SDS-PAGE gel or Western blot using these volumes (based on gel thickness of 0.75 – 1.0mm).
    • 3µl per loading on 15-well mini-gel
    • 5µl per loading on 10-well mini-gel
    • 1.5 – 2.5µl per loading for general Western transferring
    • Double the loading volume for thicker or larger gel.

ExactPro Regular Range (9-180 kDa) Prestained Protein Ladder contains 10 prestained recombinant proteins that cover a range of molecular weights from 9 kDa – 180 kDa. Proteins are covalently coupled with a blue chromophore, with 2 enhanced reference bands, 25 kDa and 75 kDA, when separated on SDS-PAGE (Tris-Glycine). Prestained proteins may have different mobilities in various SDS-PAGE buffer system.

The ladder is ready-to-use. No further dilution, addition of a reducing agent or heating is required.

Storage Buffer

20mM Tris-H3PO4 (pH 7.5 @ 25°C) 15% (v/v) Glycerol 2% (w/v) SDS 0.mM DTT 3.6mM Urea

Applications

Used in SDS-PAGE and Western blots for approximation of protein sizes, and also to locate a protein of interest for excision from an unstained preparative gel.

Recommendations

• • Thaw ladder at room temperature to dissolve precipitated solids. Do not boil.
  • Mix solution gently to ensure that it is homogeneous.
  • Load ladder into SDS-PAGE gel or Western blot using these volumes (based on gel thickness of 0.75 – 1.0mm).
    • 3µl per loading on 15-well mini-gel
    • 5µl per loading on 10-well mini-gel
    • 1.5 – 2.5µl per loading for general Western transferring
  • Double the loading volume for thicker or larger gel.

ExactPro Western Marker is a ready-to-use mixture containing 10 IgG-binding proteins with molecular weights ranging from 15 kDa to 200 kDa in Tris-Glycine Buffer. ExactPro Western Marker has 2 enhanced, reference bands (at 30 kDa and 80 kDa).

ExactPro Western Marker is compatible with chemiluminescent, fluorescent, chromogenic, and other detection systems.

The ladder is ready-to-use. No further dilution, addition of a reducing agent or heating is required.

Applications

Used in SDS-PAGE and Western blots for approximation of protein sizes, and to locate a protein of interest for excision from an unstained preparative gel. Visualization of 10 IgG-binding proteins on Western blots.

Recommendations

• Thaw ladder at room temperature to dissolve precipitated solids.
  Do not boil.
• Mix solution gently to ensure that it is homogeneous.
• Load ladder into SDS-PAGE gel or Western blot using these volumes (based on gel thickness of 0.75 – 1.0mm).
  • 5 – 2.5 µl per well for 2-step Western blot utilizing primary antibody followed by secondary antibody conjugated with reporter enzymes
  • 5 – 5 µl per well for 1-step Western blot utilizing primary antibody conjugated with reporter enzymes.
• Double the loading volume for thicker or larger gel.