ExactPro Broad Range Prestained Protein Ladder

Product Series: BIO-5150

Prestained Protein Ladder contains 13 prestained recombinant proteins that cover a wide range of molecular weights from 3.5kDa – 245kDa. An orange reference band of ~75kDa and a green reference band of ~25kDa provides easy monitoring of protein separation.

The ladder is ready-to-use. No further dilution, addition of a reducing agent or heating is required.

Storage Buffer

20mM Tris-H3PO4 (pH 7.5 @ 25°C) 15% (v/v) Glycerol 2% (w/v) SDS 10mM DTT 3.6mM Urea

Applications

Used in SDS-PAGE and Western blots for approximation of protein sizes, and also to locate a protein of interest for excision from an unstained preparative gel.

Recommendations

    • Thaw ladder at room temperature to dissolve precipitated solids. Do not boil.
    • Mix solution gently to ensure that it is homogeneous.
    • Load ladder into SDS-PAGE gel or Western blot using these volumes (based on gel thickness of 0.75 – 1.0mm).
      • 3µl per loading on 15-well mini-gel
      • 5µl per loading on 10-well mini-gel
      • 1.5 – 2.5µl per loading for general Western transferring
      • Double the loading volume for thicker or larger gel.

Interested to try it out?

Description Product No. Protocol MSDS
ExactPro Broad Range (5-245kDa) Prestained Protein Ladder, 250ul BIO-5150-250ul
ExactPro Broad Range (5-245kDa) Prestained Protein Ladder, 500ul BIO-5150-500ul
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