ExactPro Broad Range Prestained Protein Ladder

Product Series: BIO-5150

Prestained Protein Ladder contains 13 prestained recombinant proteins that cover a wide range of molecular weights from 3.5kDa – 245kDa. An orange reference band of ~75kDa and a green reference band of ~25kDa provides easy monitoring of protein separation.

The ladder is ready-to-use. No further dilution, addition of a reducing agent or heating is required.

Storage Buffer

20mM Tris-H3PO4 (pH 7.5 @ 25°C) 15% (v/v) Glycerol 2% (w/v) SDS 10mM DTT 3.6mM Urea

Applications

Used in SDS-PAGE and Western blots for approximation of protein sizes, and also to locate a protein of interest for excision from an unstained preparative gel.

Recommendations

    • Thaw ladder at room temperature to dissolve precipitated solids. Do not boil.
    • Mix solution gently to ensure that it is homogeneous.
    • Load ladder into SDS-PAGE gel or Western blot using these volumes (based on gel thickness of 0.75 – 1.0mm).
      • 3µl per loading on 15-well mini-gel
      • 5µl per loading on 10-well mini-gel
      • 1.5 – 2.5µl per loading for general Western transferring
      • Double the loading volume for thicker or larger gel.

Description Product No. Protocol MSDS
ExactPro Broad Range (5-245kDa) Prestained Protein Ladder, 250ul BIO-5150-250ul
ExactPro Broad Range (5-245kDa) Prestained Protein Ladder, 500ul BIO-5150-500ul
Online Ordering

1st BASE Online Ordering, 1oo (strongly recommended)

1st BASE Order Online simple as 1-2-3.
Try it now! It is amazingly easy to use and hassle-free!
To find out more about how to use 1oo, please click here.


For ordering or enquiries, please contact us as follow:

Singapore
Email: custcare@axilscientific.com
Tel: +65 6775 7318

Malaysia / International
Email: custcare@apicalscientific.com
Tel: +603 8943 3252

Product Description Grade Packaging Product No Product Specification Sheet Safety Data Sheet
Glycine Biotechnology Grade 500g BIO-2080-500g
1kg BIO-2080-1kg
5kg BIO-2080-5kg
Glycine Ultra Pure Grade 500g BIO-2085-500g
1kg BIO-2085-1kg
5kg BIO-2085-5kg
10X Tris Tricine Buffer Ultra Pure Grade 1L BUF-2000-10X1L
10X Tris Tricine-Sodium Dodecyl Sulfate (TT-SDS) Buffer Ultra Pure Grade 1L BUF-2010-10X1L
1X Tris Glycine (TG) Buffer Ultra Pure Grade 1L BUF-2020-1X1L
10X Tris Glycine (TG) Buffer Ultra Pure Grade 1L BUF-2020-10X1L
4L BUF-2020-10X4L
1X Tris Glycine-Sodium Dodecyl Sulfate (TG-SDS) Buffer Ultra Pure Grade 1L BUF-2030-1X1L
5X Tris Glycine-Sodium Dodecyl Sulfate (TG-SDS) Buffer Ultra Pure Grade 1L BUF-2030-5X1L
10X Tris Glycine-Sodium Dodecyl Sulfate (TG-SDS) Buffer Ultra Pure Grade 1L BUF-2030-10X1L
4L BUF-2030-10X4L
10% (w/v) Sodium Dodecyl Sulfate (SDS) Solution Biotechnology Grade 500ml BUF-2051-500ml
1L BUF-2051-1L
20% (w/v) Sodium Dodecyl Sulfate (SDS) Solution Biotechnology Grade 500ml BUF-2052-500ml
1L BUF-2052-1L