2X PCR Mastermix

Amplicon Size5kb
End ProductSticky ends
Direct Loading to GelNo
MgCl2 Concentration3mM
Amplification Rate1kb per min
Packing Size200rxn 1000 rxn
ApplicationsDNA sequencing DNA labelling PCR for cloning PCR ampli­cation of DNA fragments up to 5kb Routine PCR Colony PCR

REDiant 2X PCR Mastermix

REDiant 2X PCR Master Mix is a premixed, ready to use solution which contains Taq DNA polymerase, dNTPs, MgCl2 and reaction buffer for amplification of DNA templates by PCR. This pre-mixed formulation saves time and reduces contamination by reducing the number of pipetting steps required for usual PCR set up. The mix is optimized for efficient and reproducible PCR. It also includes a red, inert tracking dye which migrates at the same rate as 1kb DNA fragment in a 1% agarose gel.

This offer several advantages:

    • Straight forward protocol that only requires the addition of water, DNA template and primers
    • Direct loading of PCR products onto agarose gel without addition of gel loading buffer
    • Visualizes the addition of polymerase into the PCR reaction mix and ensure complete mixing
    • Acts as tracking dye in gel electrophoresis
    • Red inert dye does not inhibit PCR
Amplicon Size5kb
End ProductSticky ends
Direct Loading to GelYes
MgCl2 Concentration3mM
Amplification Rate1kb per min
Packing Size200rxn 1000 rxn
ApplicationsDNA sequencing DNA labelling PCR for cloning PCR ampli­cation of DNA fragments up to 5kb Routine PCR Colony PCR

exTEN 2X PCR mastermix

Amplicon Size10kb
End ProductMix of Sticky & Blunt ends
Direct Loading to GelYes
MgCl2 Concentration3mM
Amplification Rate1kb per min
Packing Size200rxn 1000 rxn
ApplicationsDNA sequencing DNA labelling PCR for cloning PCR ampli­cation of DNA fragments up to 10kb High sensitivity PCR High throughput PCR Routine PCR Colony PCR
Product No. Product Description Product Details Safety Data Sheet
BIO-5180-200 2X PCR Master Mix, 200 reactions
BIO-5180-1000 2X PCR Master Mix, 1000 reactions
BIO-5185-200 REDiant 2X PCR Master Mix, 200 reactions
BIO-5185-1000 REDiant 2X PCR Master Mix, 1000 reactions
BIO-5186-200 exTEN 2X PCR Master Mix, 200 reactions
BIO-5186-1000 exTEN 2X PCR Master Mix, 1000 reactions
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