Mass spectrometry (MS) is concerned with the separation of matter according to atomic and molecular mass.
High throughput MS sequencing by Electrospray is used to identify any or all molecules, differential expression analysis and also useful in full protein characterization.
For protein sequencing by LC-MS/MS, protein samples are enzymatically digested to produce fragmented peptides and going through liquid chromatography (LC) before tandem MS. Spectra are obtained for the major peptide ions in MS mode and sequence data is obtained in MS/MS mode. These spectra are then compared to databases to provide hits that identify matching or similar sequences. The end result is positive verification of the protein identity.
Analysis by LC-MS/MS is optimal for gel bands containing two or more proteins. It is also recommended for low abundant protein samples.
Samples should be submitted with our Service Order Form.