PrimeWay Gel Extraction/ PCR Purification Kit offers 2 applications in one kit. It is a simple procedure that uses a silica-based spin column to perform gel extraction or purify the DNA fragments from 65 bp to 1.5 kb within 20 minutes with a high recovery rate. This kit enables removal of primers, dNTPs, enzymes, salts, and short PCR products (< 65 bp). Thus, making it suitable for downstream processes such as DNA sequencing, PCR, in-vitro transcription, restriction mapping, cloning, and labelling applications.
FEATURES
- Fast - 10 min for PCR Purification; 20 min for Gel Extraction
- High recovery - Up to 95% for gel extraction & PCR clean-up
- Efficient - Removal of reaction contaminants including enzymes, proteins, primers dimers, dNTPs, and salts
- Convenience - No calculation required for BD buffer amount if gel slice ≤300mg
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APPLICATION DATA
High Quality DNA is Compatible for Wide Variety of Downstream Applications
including DNA sequencing, PCR, in-vitro transcription, restriction mapping, cloning, and labeling applications.
Performance Review
Comparison Data
50 ng of PCR product are analyzed using 1% TAE gel stained with Floro+Red Nucleic Acid Stain (BIO-5171-600ul). ExactMark 1 kb DNA ladder (1st BASE, BIO-5140-50ug) is used as the size standard.
40 µL of 100 bp DNA ladder (1st BASE, BIO-5130-50ug) are purified using different brands of Gel/PCR kit. DNA is eluted with 40 µL Elution Buffer.
4 µL of purified 100 bp DNA ladder are analyzed using 1.7% TAE gel stained with Floro+Red Nucleic Acid Stain (BIO-5171-600ul).
APPLICATIONS
- Suitable for downstream processes such as DNA sequencing, PCR, in-vitro transcription, restriction mapping, cloning and labelling applications.
| Product Code | KIT-9050 |
| Product | PrimeWay Gel Extraction – PCR Purification Kit |
| Size | 10/50/250 prep |
| Sample Type | Gel / PCR mixture |
| Yield/Recovery | Up to 95% |
| Purity | 1.7 - 2.0 |
| Duration | PCR Purification: 10; Gel Extraction: 20 min |
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| Size (preps) | Downloads | |
| Safety Data Sheet | 10 |  |
| 50 | |
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| 250 | |
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| Full Product Protocol | |
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| Product Profile | |
