List of Fragment Analysis Services

Product No.

Name of Service

Service Requirements

Turnaround Time

Standard Fragment Analysis Services

SS3001

Fragment Analysis with Run Report

Customers to submit fluorescently label PCR products in tube/ strips.

1st BASE to perform electrophoresis and data analysis within the expected fragment sizes.

3-4 working days

SS3002

Fragment Analysis with Run Report, 96-well format

 

Service like above (SS3001), except samples submitted in 96-well plates.

95 samples/ plate; leave H12 empty for control.

3-4 working days

SS3003

Fragment Analysis without Run Report

Customers to submit fluorescently label PCR products in tube/ strips.

1st BASE to perform electrophoresis and raw data extraction without analysis.

2-3 working days

SS3004

Fragment Analysis without Run Report, 96-well format

 

Service like above (SS3003), except samples submitted in 96-well plates.

95 samples/ plate; leave H12 empty for control.

2-3 working days

SS3012

Fragment Analysis with Run Report, without spectral optimization

 

Customers to submit properly diluted fluorescently label PCR products in tube/ strips.

1st BASE takes 2µL of the diluted DNA for electrophoresis and analyse data within the expected fragment sizes.

This is designed to meet the needs of experienced users. Dilution is typically between 1µL:3µL to 1µL:90µL (PCR template:dH20).

1 to 2 working days

SS3013

Fragment Analysis with Run Report, without spectral optimization, 96-well format

Service like above (SS3012), except samples submitted in 96-well plates.

95 samples/ plate; leave H12 empty for control.

1 to 2 working days

SS3014

Fragment Analysis without Run Report, without spectral optimization

 

Customers to submit properly diluted fluorescently label PCR products in tube/ strips.

1st BASE takes 2µL of the diluted DNA for electrophoresis and extracts raw data without analysis.

This is designed to meet the needs of experienced users. Dilution is typically between 1µL:3µL to 1µL:90µL (PCR template:dH20).

1 to 2 working days

SS3015

Fragment Analysis without Run Report, without spectral optimization, 96-well format

 

Service like above (SS3014), except samples submitted in 96-well plates.

95 samples/ plate; leave H12 empty for control.

1 to 2 working days

SS3016

FA Ready-to-load with Run Report

Customers to submit properly diluted fluorescently label PCR products with Hi-Di™ Formamide in tube/ strips.

1st BASE loads the mixture directly to Genetic Analyzer and analyse data within the expected fragment sizes.

This is designed to meet the needs of experienced users. We only accept Applied Biosystems® GS500-ROX or GS500-LIZ size standards.

1 to 2 working days

SS3017

FA Ready-to-load in 96-well format with Run Report

 

Service like above (SS3016), except samples submitted in 96-well plates.

95 samples/ plate; leave H12 empty for control.

1 to 2 working days

SS3018

FA Ready-to-load without Run Report

 

Customers to submit properly diluted fluorescently label PCR products with Hi-Di™ Formamide in tube/ strips.

1st BASE loads the mixture directly to Genetic Analyzer and extracts raw data without analysis.

This is designed to meet the needs of experienced users. We only accept Applied Biosystems® GS500-ROX or GS500-LIZ size standards.

1 to 2 working days

SS3019

FA Ready-to-load without Run Report, 96-well format

 

Service like above (SS3018), except samples submitted in 96-well plates.

95 samples/ plate; leave H12 empty for control.

1 to 2 working days

Sample Preparation and Quantification Service

SS3006

FA Spectral Optimization - Charge per first labelled primer/ locus.

 

Before loading, 3 types of Dilution Factor (DF) will be tested on the labelled sample(s) to get the optimum DF for data analysis.

To opt out of this service, please provide DF for each labelled primer.

2 working days

SS3007

FA Spectral Optimization - Charge per each add-on labelled primer.

 

Before loading, 3 types of Dilution Factor (DF) will be tested on labelled sample(s) to get the optimum DF for data analysis.

To opt out of this service, please provide DF for each labelled primer.

2 working days

SS3008

FA Spectral Optimization - Ready Multiplexed Sample.

 

Before loading, 3 types of Dilution Factor (DF) will be tested on 3 multiplexed samples to get the optimum DF for data analysis.

To opt out of this service, please provide DF for each multiplexed sample.

2 working days

SS3005

Fragment Analysis Re-Load - Single Well/ Tube

 

Where spectral optimization is not ordered together with any standard fragment analysis, each request of reload will be chargeable.

2 working days

SS3010

Fragment Analysis Sample Quantification - By Agarose Gel

This service allows customers to skip the hassle of gel electrophoresis to estimate Dilution Factor of each sample before fragment analysis. 

 

Charge per sample.

1 working day

Product No.

Name of Service

Description

Turnaround Time

Standard SNaPshot® Genotyping Services

SS3024

SNaPshot Fragment Analysis with Run Report

Customer to provide purified SNaPshot® products.

1st BASE to perform electrophoresis and data analysis.

Charge per sample.

3-4 working days

SS3022

SNaPshot® Ready-to-Load by Fragment Analysis with Run Report.

Customer to provide purified SNaPshot® product in GS120-LIZ Size Standard and Hi-Di™ Formamide.

1st BASE to perform loading directly onto Genetic Analyzer and data analysis.

We only accept Applied Biosystems® GS120-LIZ size standards ranging in 15, 20, 25,35, 50, 62, 80, 110 and 120 bp.    

Charge per load.

2 working days

SS3021

SNaPshot® reaction, clean-up and followed by Fragment Analysis with Run Report.

Customers to provide the probe sequence and the purified PCR products that contained the SNP. SNaPshot Probe® will be synthesized by 1st BASE. 1st BASE to perform SNaPshot® reaction, clean-up followed by Fragment Analysis with data analysis. 

1 week

Assay Development and Sample Preparation Services

SS3020

Singleplex SNaPshot® assay development

Customers to provide purified gDNA and SNP rs number for PCR optimization.

PCR Primers and SNaPshot Probe will be designed and synthesized by 1st BASE.    

Charge per SNP.

1 to 2 weeks

SS3020-Proviso

Singleplex SNaPshot® assay development consultation charge

Customer to provide SNP rs number for assay design.

1st BASE to design PCR Primers and SNaPshot® Probe.

Charge per SNP.

1 to 2 weeks

SS3023

PCR Reaction using the Optimized Protocol obtained from SS3020.

Customers to provide purified gDNA.

1st BASE to produce PCR product as template for SNaPshot® reaction.

Charge per PCR reaction.

3 to 5 working days

SS3025

FA Spectral Optimization - Charge per each SNaPshot® order

Before loading, 3 types of Dilution Factor (DF) will be tested on the labelled sample(s) to get the optimum DF for data analysis.

To opt out of this service, please provide DF for each SNaPshot® order.

2 working days

 

Product No.

Service Name and Description

Turnaround Time

SS3100

Human Cell Line Authentication

Genetic profiling of species specified short tandem repeat (STR) markers

Deliverable: Report and raw loci calls

3 to 5 working days