Single Pass DNA Sequencing (SS1001, SS1002)
A full service DNA sequencing reaction encompasses the cycle sequencing reactions, dye terminator removal and subsequent analysis on our Genetic Analyzers. We also accept samples in 96-well plates. This is recommended for bulk orders, with a minimum of 80 reactions per plate.
Customers need to send us their purified DNA (plasmid or PCR product) and specific primers individually. We strongly recommend 1.5mL of microtube to be used for Service Code SS1001; and PCR strip-capped 96-well plate for Service code: SS1002
Sample Preparation Requirements for DNA Sequencing Reactions for local customers (Singapore and Malaysia)
|
Type of DNA Template |
Recommended Concentration/ Reaction |
Required Volume/ Reaction |
|
Purified PCR Product [90-500bp] |
5 ng/µL |
10 uL |
|
Purified PCR Product [501-1500bp] |
20 ng/µL |
|
|
Purified PCR Product [>1500bp] |
30 ng/µL |
|
|
Purified Plasmid (< 4kb) |
50 ng/µL |
|
|
Purified Plasmid (4kb to 10kb) |
80 ng/µL |
|
|
Purified Plasmid (>10kb) |
100 ng/µL |
|
|
Purified M13/ phagemid ssDNA |
50 ng/µL |
|
|
Purified Genomic DNA/ BAC |
400 ng/µL |
20 uL |
Type of Primer |
Required Concentration |
Total of Reaction |
Required Volume |
Sequencing Primer
|
10µM or 10pmol/µL or 50ng/uL |
1 |
10uL |
10 |
25uL |
||
20 |
50uL |
Sample Preparation Requirements for International Customers
We recommend that international customers send a larger volume of their samples to overcome loss of samples due to evaporation during the extended transportation to our facilities.
|
Type of DNA Template |
Recommended Concentration/ Reaction |
Required Volume/ Reaction |
|
Purified PCR Product [90-500bp] |
5 ng/µL |
15 uL |
|
Purified PCR Product [501-1500bp] |
20 ng/µL |
|
|
Purified PCR Product [>1500bp] |
30 ng/µL |
|
|
Purified Plasmid (< 4kb) |
50 ng/µL |
|
|
Purified Plasmid (4kb to 10kb) |
80 ng/µL |
|
|
Purified Plasmid (>10kb) |
100 ng/µL |
|
|
Purified M13/ phagemid ssDNA |
50 ng/µL |
|
|
Purified Genomic DNA/ BAC |
400 ng/µL |
25 uL |
Type of Primer |
Required Concentration |
Total of Reaction |
Required Volume |
Sequencing Primer
|
10µM or 10pmol/µL or 50ng/uL |
1 |
10uL |
10 |
25uL |
||
20 |
50uL |
To view list of complimentary Universal Primers provided by our DNA Sequencing facility, please click here.