As the COVID-19 virus continues to spread, communities across the globe are struggling to keep up with the demand for public testing but also research discoveries pertaining to Sars-CoV-2.
The need is urgent and 1st BASE is here to assist you with them.

Browse through for the available solutions for COVID-19 from 1st BASE.

 

 

 

Sanger Sequencing Services

1st BASE Sanger Sequencing provides the required information to distinguish COVID-19 from other respiratory pathogens and in identifying new mutations within the genes.

Get in touch with us for your sequencing requirements!

  • Priority Sequencing
  • High-quality sequencing
  • Tap on our expertise with sound technical support

M13-Tailed Sequencing (SS1101)

* Complementary PCR optimization

Sample type: Unpurified qPCR product

  • Consistent sequencing results for COVID-19 qPCR amplicons.
  • Includes quantification of templates, M13-tail PCR, PCR product purification, Bi-directional sequencing, and alignment to reference sequence.

Single Pass DNA Sequencing(SS1001, SS1002)

Sample type: Purified PCR product / Plasmid

  • DNA sequencing reaction encompasses the cycle sequencing reactions, dye terminator removal and subsequent analysis on our Genetic Analyzers.

Sequencing+ PLUS (SS1201)

Sample type: Unpurified PCR product / Excised Gel Band

  • All-in-one service.
  • Includes purification / gel extraction, and Single Pass DNA Sequencing.

 

 

 

Custom Manufacturing of Biochemicals, Buffers & Reagents

Apart from our extensive list of ready-to-use buffers and media, 1st BASE is also open to customization of project/products, from packaging size to formulation and testing, tailored to your needs.

Get in touch with us for your custom request!

 

1st BASE Viral Transport Medium (VTM)

Viral Transport Medium (VTM) is a pH-buffered fluid with a specific formulation of salts, protein and other chemicals that can maintain the genetic information of the virus prior testing.

1st BASE VTM is formulated based on CDC Atlanta standards. 

Product Code

Item Description

Storage

PKG Size

CUS-7020-1L

Viral   Transport  Media  (VTM),

Biotechnology Grade.

4°C

1L

CUS-7020-4L

4L

 

1st BASE Viral DNA/RNA Extraction Kit

The kit allows for enhanced extraction of viral DNA/RNA (nucleic acid) from serum, plasma, body fluids, cell culture supernatant and viral transport medium swabs. This is a column-based extraction method that utilizes silica membrane spin column technology where the procedure involves lysis of viral cells, optimum binding of viral nucleic acid onto the silica membrane, washing and finally eluting viral nucleic acid from the silica membrane. The end-user is able to purify their viral DNA/RNA in less than 30 minutes. Product insert as attached.

A special feature of our kit is that it includes an inert carrier [poly-A] powder which enhances the binding of viral nucleic acid onto the silica membrane to improve its binding efficiency. It is an ideal kit to isolate low viral copies from 201 to 207 copies of viral nucleic acid. The purified viral nucleic acid is optimum for RT-PCR and qRT-PCR applications.

Product Code

Item Description

Storage

PKG Size

BIO-9010-50

Viral DNA/RNA Extraction Kit

RT

50 preps/kit

 

1st BASE 2019-nCoV Plasmid Positive Controls

The 2019-nCoV Plasmid Positive Controls are packaged for 500µL (200,000 copies/µL) in IDTE pH 8.0. This control will be ideal to existing COVID-19 screening users that are working on the E Sarbeco and RdRP genes. Each tube is sufficient for 2,500 duplicate reactions.

The product comes with one circular plasmid DNA, which contains partial sequences of 2019-nCoV E Sarbeco gene, RdRP gene and RNAse P gene.

As compared to our first version of positive control (Product No: MBS-4100) that was designed solely to be the positive control in Charité/Berlin qPCR protocol (version 17th Jan 2020), the version 2 of positive control includes new fragments of RdRP Helicase and human RNAse P for qPCR detection assay according to Chan JF-W et al., 2020 and CDC assays for SARS-CoV-2 detection respectively. Human RNAse P gene is regularly used as internal control (IC) for qPCR assay of SARS-CoV-2 detection.

Furthermore, we have introduced a pair of modified qPCR primers (Product No: CV013 and CV014) to detect RdRP gene using the same RdRP P2 probe in Charité/Berlin protocol. This resolves the assay sensitivity to better distinguish the signal between NTC and RdRP gene.

Product Code Item Description Manual or Protocol Product Specification Sheet Safety Data Sheet
MBS-4100 2019-nCoV_E_RdRp Positive Control Download Download Download
MBS-4101 2019-nCoV_Positive Control_v2 Download Download Download

Get in touch with our team of molecular biology experts for your requirement!

 

 

 

 

 

Next-Generation Sequencing Services for SARS-CoV-2 Genome 

NGS enables the study of genomic epidemiology and evolution of SARS-CoV-2 where the viral genome data generated prospectively during outbreaks can help provide information about relatedness to other viruses, mode and tempo of evolution, geographical spread and adaptation to human hosts. It also provides crucial elements of coronavirus pathogenicity and possible targets for diagnostics, prognostication and interventions.

 

As country-specific SARS-CoV-2 datasets are still limited, there has been much interest and effort being taken to sequence the genome of the SARS-CoV-2. Let us help support you with the genome sequencing of the virus.  

Get in touch with our team of experts! 

  • Priority Sequencing
  • All-in-one Package: From Total RNA to Bioinformatics Analysis
  • Guaranteed Sequencing Output: 330x (10Mb) per Sample


Specification of Service: 

 

 

 

 

Molecular Biology Service

As facilities race to ramp-up capacity, supply shortages, not just personnel and lab space shortages, have waylaid some testing efforts.

Let us help you with the processing steps of your COVID-19 experiments.  

Get in touch with our team of molecular biology experts for your requirements! 

  • All-in-one Solution: Samples to reporting
  • Maximise resource efficiency: Saves you precious time to focus on important issues
  • Quality You Can Count On: Robust Protocols That Ensure Superior Data