Keep your experiments safe with RNase Quiet, the ready-to-use solution that eliminates RNase, DNase, DNA, and RNA contamination in seconds. From glassware to plastic tools and lab benches, it ensures your workspace stays nuclease-free for accurate, reproducible results.
- Effective Removal of Nucleases and Nucleic Acids
- Simple spray-and-wipe application
- No detergent needed - quick and hassle-free
- Safe to use: non-carcinogenic, DEPC-free
Application
Fig 1. Fidelity comparison across commercially available products.
Condition
- Apply 100 μl RNase A solution (1 mg/ml) to cover glasses and dry them.
- Spray with DEPC-treated water or RNase Quiet and wait for 1 minute. Wipe thoroughly with a clean paper towel, then rinse with RNase-free sterile water.
- Apply 50 μl RNA solution (40 μg/ml) on the cover glasses and incubate them at 37°C for 30 minutes.
- Apply 1 μl ethidium bromide solution (20 μg/ml) to the cover glasses with a pipette.
- Observe with UV.
Application 2: RNase and DNase decontamination of 1.5 ml micro-tubes
Condition
- RNase decontamination test
(+): Add 10 μl of RNase A solution (10 mg/ml) to a 1.5 ml micro-tube.
(−): Add 10 μl of lysate bu er to a 1.5 ml micro-tube. - DNase decontamination test
(+): Add 10 μl of DNase I (27.3 U/10 μl) to a 1.5 ml micro-tube, and dry it up.
(−): Add 10 μl of lysate bu er to a 1.5 ml micro-tube, and dry it up. - Add 1 ml of DEPC-treated water or RNase Quiet and wait for 1 minute.
- Remove the solution from the tubes and rinse those with 1 ml of DEPC-treated water.
- Remove the DEPC-treated water from the tubes and add 25 μl of RNA solution (40 μg/ml) and 1 μl of 50 mmol/L magnesium chloride for RNase decontamination test; 25 μl of DNA solution (40 μg/ml) and 1 μl of 50 mmol/L magnesium chloride for DNase decontamination test, and incubate them at 37°C for 30 minutes.
- Analyze them using electrophoresis with 1% agarose gel including ethidium bromide.
Application 3: Decontamination Efficiency
- Add 2.5 μl of RNA solution (0.4 mg/ml) or 2.5 μl of DNA solution (0.4 mg/ml) to 1.5 ml micro tubes.
- Add each concentration of RNase Quiet solution as mentioned above and 20 μl of DEPC treated water into a tube, and then incubate them for 5 minutes.
- Analyze them with agarose gel electrophoresis with ethidium bromide.
| Product Description | Storage | Product No. | Pack Size | Product Manual | Safety Data Sheet |
| RNase Quiet (with spray nozzle) | Room Temp | BIO-6010-475ml | 475ml |  |
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