T7E1 Mismatch Cleavage Assay

The mismatch cleavage assay is a simple and cost-effective method to detect a mutation or variation within a targeted region. The targeted region is first amplified, and the amplified products are simultaneously digested with T7E1 enzymes to cleave heteroduplex DNA at mismatches or extrahelical loops formed by multiple nucleotides, yielding two or more smaller fragments. T7EI is able to recognise insertions and deletions (Indels) of ≥ 2 bases that are generated by non-homologous end joining (NHEJ) activity in CRISPR experiments.