Services Offered

Product No. Name of Service Service Includes Suited for
Protein Identification Services
SS001-1 MALDI-TOF/TOF mass spectrometry (Sequencing by MS/MS) Trypsin digestion of protein, running of samples with automatic database analysis. Optimal for pure protein from 2D gel spots
SS001-2 Electrospray (LC/MS/MS) Mass Spectrometry
Trypsin digestion of protein, running of samples with automatic database analysis.
Optimal for a single gel band containing 2 or more proteins, or low abundant samples.
SS002 De Novo Peptide Sequencing by Mass Spectrometry. MALDI TOF/TOF is used to determine the primary sequence structure of proteins. Manual interpretation of the MS/MS spectra and derivation of internal sequence will be done using the latest software.
Optimal for pure protein from 2D gel spots, and does not have the related genome from database.
SS002-1 De Novo Peptide Sequencing by Mass Spectrometry.

MALDI TOF/TOF is used to determine the primary sequence structure of proteins. Manual interpretation of the MS/MS spectra and derive of internal sequence will be done by the expert operator using the latest software. Price per sample inclusive of 1 peptide sequence for de novo search.

Optimal for 2D gel spot with coomassive stain only and   available only for pure protein stained with Coomasive blue, that does not have the related genome from database.
SS002-2 De Novo Peptide Sequencing by Mass Spectrometry.

LC/MS/MS  is used to determine the primary sequence structure of proteins. Manual interpretation of the MS/MS spectra derived from internal sequence will be done by the expert operator using the latest software. Price per sample inclusive of 1 peptide sequence for de novo search

Only available for pure protein stained with Silver or other MALDI compatible stains eg.Ruby or Cy dyes, that does not have the related genome from database.
SS002-3 De Novo Peptide Sequencing, for each additional peptide.

Additional peptide sequence needed for De Novo Peptide Sequencing Service

For de novo samples that require probe/primer design, a minimum of 3 peptide sequences for de novo search is recommended.
SS010-1 N-Terminal Protein Sequencing, first 3 amino acid residues. Edman degradation. The first 3 amino acid residues is also the basic fee if the N-terminal is blocked (which is the no result fee). Pure proteins that require definitive sequence of the amino acid from the N-terminus.
SS010-2 N-Terminal Protein Sequencing, each additional amino acid residues Edman degradation. Minimum 12 residues are recommended for protein identification. 15 residues are highly recommended (up to 30 residues)
Proteome Mapping – MuDPIT Analysis
SS003-1
Proteome Mapping – MuDPIT analysis: 1D LC-MALDI. Price per Sample.
Simple protein sample is run through an extended LC gradient, and the eluent spotted for MALDI-TOF/TOF analysis. Optimal for simple proteins. It allows identification up to 100 spots per experiment.
SS003-2 Proteome Mapping – MuDPIT analysis: 2D LC-MALDI. Price per Sample. Complex protein sample is run through a sophisticated 2D LC gradient, and the eluent spotted for MALDI-TOF/TOF analysis. Optimal for complex proteins. It allows identification up to1000 spots per experiment.
Protein Sample Preparation Services
SS004-1 2-Dimensional gel electrophoresis. Large gel at price per gel. Including 1st, 2nd dimension gels and staining (silver/ Coomassie) Crude proteins usually acquire 4 gels per experiment.
SS004-2 2-Dimensional gel electrophoresis. Small gel at price per gel. Including 1st, 2nd dimension gels and staining (silver/ Coomassie)
SS004-3 2-Dimensional gel electrophoresis service: High sensitivity Sypro staining. Price per gel. High sensitivity of Sypro staining. For weak samples that require staining others than Coomassive and Silver staining.
SS004-4 2-Dimensional gel electrophoresis service: Image analysis and spot recognition (Client gels) by Progenesis Same Spots. Set of four replicate gels. Price per gel. Image analysis and spot recognition Recommended for crude proteins that require both 2D services and image analysis.
SS004-5 2-Dimensional gel electrophoresis service: Pattern comparison. Two sets of four replicate gels. Price per set. Gel replicates merged to prepare master gels, which are compared in pairwise sets. Recommended for crude proteins that require both 2D services and image comparison between other protein(s) that submitted together.
SS004-6 2-Dimensional gel electrophoresis service: Blotting of protein from 2D PAGE gels onto sequencing grade PVDF membrane. Price per blot. We now can accept protein samples from 2D samples that run by us for N-terminal protein sequencing, without the need of blotting them onto PVDF membrane.
This is only recommended for the customer that needs their protein to be run on 2D, and return the protein on PVDF membrane.
Protein Differential Expression – iTRAQ Analysis

SS005-1
iTRAQ Enhanced Analysis Service

Sample labelling and analysis by

(i) 1D LC-MALDI mass spectrometry to understand the sample complexity

(ii) 2D LC-MALDI mass spectrometry, with automatic database analysis (replicate, up to 4 samples)

 

Price excludes dry-ice shipment.

Minimum 2 different protein extracts for one iTRAQ analysis (1 protein extract is the control/ reference, another 1 protein extract is the targeted sample for differential expression comparison)
SS005-1-Proviso Minimum setup for iTRAQ Enhanced Analysis Service Setup fee will apply if sample(s) failed to proceed to 2D LC-MALDI mass spectrometry. Price excludes dry-ice shipment  
SS005-2 iTRAQ  Analysis Service (Re-run) Sample labelling and analysis by 2D LC-MALDI mass spectrometry, with automatic database analysis.

 

Price for repeat experiment from SS005-1 (replicate, up to 4 samples), excludes dry-ice shipment.

Proteins that are extracted in the different period may have different expression profile. This service is recommended for re-run of same protein extracts that were already analysed in SS005-1.
Protein Mass and Component Analysis
SS006-1 Multiple Reaction Monitoring (MRM) analysis: Method development. Price per day. Estimate 2 to 5 days are required for each method.
Biomarker analysis and reaction monitoring in pharmacokinetic studies for toxic metabolites.
SS006-2 Multiple Reaction Monitoring (MRM) analysis: Data collection and analysis. Price per sample Data collection and analysis performed as agreed in the project/ method from SS006-1.  
SS007 Phosphorylation Detection. Price per sample Precursor ion and neutral loss scans that allow detection of the loss of the phosphate group. Pure protein and peptide samples required identification of phosphorylation sites. These sites can be mapped to detect the important post-translational modification of proteins.
SS008-1 Protein Mass analysis: peptide (<15 kDalton). Price per peptide. Samples are processed intact (no enzyme digestion). Intact mass by MALDI-TOF mass spectrometry.
Pure peptide (<15 kDalton). Most of the peptide has mass < 15 kDalton.
SS008-2 Protein Mass analysis: protein (<15 kDalton). Price per protein. Samples are processed intact (no enzyme digestion). Intact mass by MALDI-TOF mass spectrometry. Pure protein (<15 kDalton).
SS008-3 Protein Mass analysis: peptide (>15 kDalton). Price per peptide.
Samples are processed intact (no enzyme digestion). Intact mass by electrospray mass spectrometry.
Pure peptide (>15 kDalton)
SS008-4 Protein Mass analysis: protein (>15 kDalton). Price per protein. Samples are processed intact (no enzyme digestion). Intact mass by electrospray mass spectrometry. Pure protein (>15 kDalton). Most of the protein has mass > 15 kDalton.
SS009-1 Amino Acid Analysis: Peptide. Price per sample. Chemical hydrolysis up to 18 amino acids per analysis. Not included Cystein and Tryptophan detection. Ideal for quality control and the amino acid composition of peptides.
SS009-2 Amino Acid Analysis: Protein. Price per sample. Chemical hydrolysis up to 18 amino acids per analysis. Not included Cystein and Tryptophan detection. Ideal for quality control, the amino acid composition of protein and peptide samples (include recombinant proteins from cell culture and fermentation material, nutraceuticals and plant materials).
SS009-3 Amino Acid Analysis: Determination of Cystein per analysis. Price per sample. Special hydrolysis method to detect only Cystein. Peptide/ protein sample(s) that specified for Cystein detection.
SS009-4 Amino Acid Analysis: Determination of Tryptophan per analysis. Price per sample. Special hydrolysis method to detect only Tryptophan. Peptide/ protein sample(s) that specified for Tryptophan detection.
Others
- Project Consultation - Please enquire