Q1) Can you extract bacterial gDNA for next generation sequencing (NGS)?
Ans: Yes. Please ship the bacteria culture to us in agar slant and also enough culture broth powder to make 500 ml liquid broth at room temperature. We will culture the bacteria and perform gDNA extraction. This is followed by bacterial 16S rRNA (partial sequence 600 bp) PCR to confirm the identity of bacteria.
Q2) How to ship purified gDNA for NGS?
Ans: It is compulsory to ship at 4oC or blue ice. If you need certified endotoxin free plasmid prep, please enquire.
Q3) How to ship bacterial culture for plasmid extraction?
Ans: You can send bacteria culture in stab agar or agar slant at room temperature. We don’t encourage our customer to ship bacteria culture on agar plate. This is because agar plate might be damaged during courier shipping. Please indicate the preferred antibiotics for growing bacteria as well.
Q4) Can you remove the RNA contamination of gDNA?
Ans: Yes. Please enquire.
Q5) What is the grade of plasmid (midiprep, maxiprep) delivered?
Ans: We use commercial kit to purify plasmid. The plasmid is ultrapure, transfection grade. It is NOT certified endotoxin-free. If you need certified endotoxin free plasmid prep, please enquire.