Species Barcoding

Utilising our expertise in DNA sequencing, 1st BASE now offers barcoding/identification services for pure isolates of bacterial, fungal, plant and animal samples. Molecular methods will be employed to give more accurate and reproducible results and even identifying the samples down to the genus level.

Appropriate controls are used at all levels to ensure the results are consistent and of the highest quality. All reports include the assembled sequences of your samples and the alignment of the top 10 hits to GenBank databases. 

For other species, please enquire.

Features

  • Barcoding and identification services for isolated pure samples
  • Accurate and reproducible results
  • Fast turnaround time
  • Bidirectional sequencing prevents ambiguity in sequence
  • Sequences are matched against a standard database, or a database of choice
  • Comprehensive reports include sequences of sample, and the top 10 Hits of the BLAST results
  • Additional analyses e.g. Phylogenetic analysis available
  • NEW Free Neighbor Joining (unrooted tree)

Protocol

  1. PCR Amplification of conserved regions of gDNA sample
  2. Purification of amplicons
  3. Bi-directional sequencing of amplicons
  4. Data alignment and BLAST of results

Specifications

Product No.

Description

MBS-5001

Bacterial Species Barcoding, v4-v6 region of  16s rRNA (~ 600bp) 

Includes:

  • Extraction of gDNA, PCR amplification and Purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database)

MBS-5002

Bacterial Species Barcoding, full length of 16s rRNA (~ 1400bp) 

Includes:

  • Extraction of gDNA, PCR amplification and Purification
  • Bidirectional PCR product sequencing  
  • Data analysis (BLAST to show the top 10 matches from database)

MBS-5003

Plant Species Barcoding, rbcl (~ 600bp) and matK (~850 bp) 

Includes:

  • PCR amplification and purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database)

MBS-5004

Fungal Species Barcoding ITS (~ 700bp)  

Includes:

  • PCR amplification and purification
  • Bidirectional PCR product sequencing
  • Data analysis (BLAST to show the top 10 matches from database)
MBS-5005

Animal Species Barcoding, COI (~ 700bp)

Includes:

  •  PCR amplification and purification
  • Forward Single Pass Sequencing of cloned DNA
  • Data analysis (BLAST to show the top 10 matches from database
MBS-5006 NEW

Eukaryote Species Barcoding, 18s rRNA (~ 1800bp)

Includes:

  • PCR amplification and purification
  • Primer walking of Cloned DNA
  • Data analysis (BLAST to show the top 10 matches from database)
MBS-500x - Proviso

Species Barcoding - Pure gDNA isolate from bacteria, fungus, plant or animal

A minimum set-up fee is chargeable when there is no PCR result.

MBS-5101 

Bacterial Species Barcoding from mixed isolates, 16 sRNA, partial sequence (~ 600 bp). Includes 
+ Cloning and 8 positive clones send for Forward Single Pass Sequencing
+ Data analysis (Alignment & BLAST to show the top 10 matches from database).

MBS-5102 

Bacterial Species Barcoding from mixed isolates, 16 sRNA, full-length sequence (~ 1500 bp). Includes

+ Cloning and 8 positive clones send for Bidirectional Sequencing

+ Data analysis (Alignment & BLAST to show the top 10 matches from database).
MBS-5104 

Fungal Species Barcoding from mixed isolates, ITS (~ 700 bp).

Includes

+ Cloning and 8 positive clones send for Forward Single Pass Sequencing

+ Data analysis (Alignment & BLAST to show the top 10 matches from database).
MBS-5200

 

Species Barcoding

 

gDNA mini prep for plant, bacteria, fungus (Solutions A and B will be provided)

MBS-5201

 

Species Barcoding

gDNA mini prep for animal (Solutions A, TP and B will be provided)