Sub-cloning of Gene Construct (up to 1.5kb) to a vector of choice

At 1st BASE, we offer a sub-cloning service, where customers may provide their gene construct as well as their destination vector. This service includes subcloning of an insert from its original holding plasmid or a gene synthesis product into customer supplied vector, colony PCR screening and DNA sequencing for confirmation. We will deliver the new Gene Construct up to 10µg as lyophilized DNA. This service also includes sequencing data and Service Report.

Specifications

 Product No.

Description

MBS-3001 NEW

Subcloning Service (up to 1.5kb)
Includes subcloning of an insert from its original holding plasmid or a gene synthesis product into customer supplied vector, colony PCR screening and DNA sequencing for confirmation.
MBS-3001-Proviso

Subcloning Service
Minimum set-up fee is chargeable when no positive clone is obtained after picking up to 24 clones with control. Any insert more than 1.5kb, surcharge of Primer Walking will be apply.

The table below lists a limited selection of non-standard vectors that our Molecular Biology Service (MBS) team that have used in this sub-cloning service. Due to intellectual property rights, we do not stock any non-standard vectors. Instead, customers will need to provide the destination vector to us. Please enquire if you would like to work with non-standard vectors that are not listed within this table: 

Vector name

Vector size

Resistance

Primer sites 

pcDNA3.1-HisA, B, C

5514

AmpR

T7,BGH rev

 

pBluescriptIISK(+)

2961

AmpR

M13+/M13-, T7, T3

 

pGEM-T easy

3015

AmpR

M13+/M13-, T7, SP6

 

pCAMBIA1301

11849

KanR

Custom designed

 

pQE30

3461

AmpR

Custom designed

 

Please complete our Sub-cloning Service Form in order to process your order or enquiry.

Sample Submission Requirements:

  1. ≥ 0.5 µg of original holding plasmid DNA (>10 ng/µl), ≥ 4 µg of Destination plasmid DNA (>150 ng/µl) in either TE or 10mM Tris-HCl (pH8.0 - 8.5) of Elution Buffer (e.g. QIAGEN EB Buffer). Supplied DNA must meets OD 260/280= 1.8 to 2.0
  2. Attached Gel Photo - 2 µl of DNA should show a bright band. Please indicate DNA ladder, amount/volume of DNA on gel, target band.
  3. Samples submitted in 1.5ml microcentrifuge tubes with at least 10 µl of contents. Each tube should be clearly labeled using a permanent marker, with caps sealed with parafilm. DNA should be shipped chilled.

Note: 1st BASE will perform verification on sample quality and integrity upon receipt before proceeding with any sub-cloning service.