PCR Product Cloning

1st BASE offers PCR Product Cloning service to clone either purified or unpurified PCR product into our standard pBASE-1 or pJET1.2 /Blunt vectors. Screening for positive clones and DNA sequencing will be done after cloning for confirmation of positive clone containing full length inserts.

Specifications

Product no

Description

MBS-3002 NEW

PCR Product Cloning Service (up to 1.5kb)

Includes cloning of a purified / unpurified PCR product into pJET1.2/ Blunt or pBASE-1 vector, colony PCR screening and DNA Sequencing for confirmation.

MBS-3002-Proviso A minimum set-up fee is chargeable when no positive clone is obtained after picking up to 8 clones with control.

MBS-3003

PCR Product Cloning Service (3kb to 5kb)

Includes cloning of a purified/ unpurified PCR product into pJET1.2 /Blunt or pBASE-1 vector, colony PCR screening and DNA sequencing for confirmation.

MBS-3003-Proviso

A minimum set-up fee is chargeable when no positive clone is obtained after picking up to 8 clones with control.

MBS-3004

Amplify and Purify PCR Products Prior to Cloning or Sub-cloning Services

Customer to provide PCR protocol and DNA template. Price does not include primer synthesis or sequencing.

MBS-3005 Additional screening for positive clone. Price per screen (24 colony picked).
MBS-3006 PCR Product Cloning Service PLUS (up to 1.5kb) *5 positive clones sent for colony PCR product sequencing.