Site-Directed Mutagenesis

Site-Directed Mutagenesis (SDM) is combination of techniques from DNA synthesis and advanced-PCR. The final construct will eventually cloned into a desired vector for the ease of sequence verification by Sanger sequencing.

One mutation is defined as any combination of mutation within the frame of 50 bases at any desired site. In the end of the final construction, it is however up to the researcher whether they want to verify the entire inserted sequence using primer walking or only sequence the mutation sites.

Specifications:

Product Number

Description

MBS-8006 NEW

Site-directed Mutagenesis (SDM). Price per mutation (each 50 bases).

Includes:

Design and synthesis of PCR primers

Mutagenesis of target gene, subclone to customer provided vector

Sequencing result

Sample requirements:

  1. Please submit ≥ 1µg of original holding plasmid DNA (>150 ng/µl) in either TE or 10mM Tris-HCl (pH8.0 – 8.5)
  2. Please submit ≥ 4µg of destination plasmid DNA (>150 ng/µl) in either TE or 10mM Tris-HCl (pH8.0 – 8.5)