Primer Walking for Large Insert (>= 7kb)

This service is an expansion of our regular primer walking (http://www.base-asia.com/dna-sequencing-services/services-offered/primer-walking) for insert sizes greater than 7kb.

There are 2 options in primer walking; Single Pass or Bi-directional. See www.base-asia.com/dna-sequencing-services/services-offered/primer-walking for details.

The base pair charges start from the first until the last base of the inserted fragment in the holding vector. As such, customer is required to provide vector name and RE cut sites (if this applies) for sequence assembly.

 

Specifications:

Product No.

Description

MBS-8001

Single Pass Primer Walking

  • Free plasmid miniprep for insert size >=3kb
  • Primer design and synthesis
  • Sequencing using designed primer
  • Assembly and sequence alignment


Price per base (insert size).

MBS-8002

Bi-directional Primer Walking

  • Free plasmid miniprep for insert size >=3kb
  • Primer design and synthesis
  • Sequencing using designed primer
  • Assembly and sequence alignment

Price per basepair (insert size)

Sample requirements: Purified plasmid DNA at 100 ng/µL, min 50 µL in dH2O