Primer Walking > 7kb

This service is an expansion of our regular primer walking (http://www.base-asia.com/dna-sequencing-services/services-offered/primer-walking) for insert sizes greater than 7kb.

There are 2 options in primer walking; Single Pass or Bi-directional. See www.base-asia.com/dna-sequencing-services/services-offered/primer-walking for details.

Specifications:

Product Number

Description

MBS-8001

Single Pass Primer Walking > 7kb, price per project.

Includes:

Plasmid Preparation
Primer design & synthesis
Sequencing using designed primer
Assembly and sequence alignment

MBS-8002

Bi-directional Primer Walking > 7kb , price per project.

Includes:

Plasmid Preparation
Primer design & synthesis
Sequencing using designed primer
Assembly and sequence alignment

The base pair charges start from the first until the last base of the inserted fragment in the holding vector. As such, customer is required to provide vector name and RE cut sites (if this applies) for sequence assembly.

Sample requirements:

Please submit purified plasmid DNA at 100 ng/µL, min 50 µL in dH2O