Genome Walking

Genome walking is a method for walking upstream or downstream (e.g. towards promoter regions) of genomic DNA from a known sequence, such as gene of interest (GOI) or transgene.

It is important that the provided known sequence was verified using Sanger sequencing before the genome walking. As such, the first round of sequencing reactions using the provided PCR Primers of the GOI have to be successful and sequence verified before we may proceed with the Genome Walking project.

 

Specifications:

Product Number

Description

MBS-8005 (N=1kb)

Genome Walking:
Up to 1kb upwards & downwards flanking the known GOI.

  • Validation of the presence of GOI
  • Primer Synthesis
  • PCR, Cloning & Sanger Sequencing


Price per project.

Sample requirements: ≥ 10µg of purified gDNA (RNA-free).