Fragment Assembly

Fragment assembly joins multiple overlapping double stranded DNA fragments. In the end of the fragment assembly, the final construct will be cloned into a holding plasmid and sequence verified by Sanger sequencing.

This method is widely used during construction of longer genes, where synthesis of a long gene is chemically challenging and expensive.

Two services are offered as below:

  1. Assembly of IDT gBlocks TM into pBASE-1 vector
    • This includes fragments design and assembly, cloning and sequencing of the final construct.
  2. Assembly of dsDNA fragments (PCR products and synthetic genes)
    • This includes fragments design and assembly, primer synthesis, PCR, cloning and sequencing of the final construct.

The green bar of the vector map below illustrated the position of the final construct into our free holding vector, pBASE-1.

Specifications:

Product Number

Description

MBS-8004 NEW

Fragment Assembly , price per project.

Includes:

Fragment Assembly

Cloning

Sequencing of insert