PCR Product Cloning

We offer PCR Product Cloning service to clone unpurified PCR product into pJET1.2/Blunt vectors.

For PCR Product Cloning Services, screening of positive clones will be done and only 1 of the positive clone containing full length insert will send for sequencing for sequence confirmation. If you need to sequence more than 1 positive colony, we offer PCR Product Cloning Service PLUS services to sequence 5 positive colonies.

Minimum setup fee will apply when no positive clone obtained after picking of up to 8 clones with control.

Additional screening of positive clones is also available at separate cost.

 

Specifications

Product No.

Description

MBS-3002 

PCR Product Cloning Service (up to 1.5kb)
Includes: Cloning of un-purified PCR product into pJET1.2™/ Blunt vector, colony PCR screening and DNA sequencing for confirmation.

MBS-3005

Additional screening for positive clone. Price per screen (24 colony picked).

MBS-3006

PCR Product Cloning Service PLUS (up to 1.5kb)
Includes: Cloning of un-purified PCR product into pJET1.2™/ Blunt vector, colony PCR screening and pick 5 positive colony PCR products for sequencing (1xFor & 1xRev) confirmation.

SS1008

Primer Walking of Constructs Service - Single Pass

Surcharge for gene construct > 1.5kb. Price per base.

Note: pJET1.2™ is the registered trademark from Life Technologies and Thermo Fisher Scientific.

 

Subcloning of Gene Construct 

We offer a subcloning service, where customers may provide their gene construct as well as their destination vector. This service includes subcloning of an insert from its original holding plasmid or a gene synthesis product into the customer choice of destination vector, colony PCR screening and DNA sequencing for confirmation.

We will perform verification on quality and integrity upon receipt of original sample(s) before proceeding with any subcloning service.

Minimum setup fee will apply when no positive clone obtained after picking of up to 24 clones with control.

Due to intellectual property rights, our subcloning services does not include the cost of destination vector. Instead, customers will need to provide us the destination vector separately upon order confirmation.

The final deliverables include the new gene construct as lyophilized DNA, together with its sequencing data and a service report.

 

Specifications

 Product No.

Description

MBS-3001 

Subcloning Service (up to 1.5kb of insert)
Includes: Subcloning of an insert from its original holding plasmid into other destination vector, colony PCR screening and DNA sequencing for confirmation.
SS1008

Primer Walking of Constructs Service - Single Pass

Surcharge for gene construct > 1.5kb. Price per base.

 

 

Fragment Assembly

Fragment assembly joins multiple overlapping double stranded DNA fragments. In the end of the fragment assembly, the final construct will be cloned into a holding plasmid and sequence verified by Sanger sequencing.

This method is widely used during construction of longer genes, where synthesis of a long gene is chemically challenging and expensive.

Two services are offered as below:

  1. Assembly of IDT gBlocks™ into pJET1.2™/ Blunt vector
    • This includes fragments design and assembly, cloning and sequencing of the final construct.
  2. Assembly of dsDNA fragments (PCR products and synthetic genes)
    • This includes fragments design and assembly, primer synthesis, PCR, cloning and sequencing of the final construct.

Note1: gBlocks™ is the registered trademark from Integrated DNA Technologies (IDT)

Note2: pJET1.2™ is the registered trademark from Life Technologies and Thermo Fisher Scientific.

 

Specifications

 Product No.

Description

MBS-8004 (N=x) 

Fragment Assembly. Number of fragments = x. 

  • Fragment Assembly
  • Cloning
  • Sequencing of insert up to 1.5kb

Price per project.

SS1008

Primer Walking of Constructs Service - Single Pass

Surcharge for gene construct > 1.5kb. Price per base.