HRM (High Resolution Melting)

HRM is a molecular biology technique used to detect mutations, polymorphisms and epigenetic differences in double-stranded DNA. It is a PCR-based method capable of discovering small differences such as indels or SNPs through high resolution melting curve analysis. If the location of a mutation or variant is unknown in a population study, HRM could be a good choice to categorise individuals based on high resolution melting curve analysis followed by sequencing to verify its sequence.