How to Order

To place an order or make an enquiry, please contact your Product Specialist or you may send in your order requests to the following email addresses based on your location.

 

Location Singapore Malaysia International
Email to: ngs@
axilscientific.com
ngs-my@
base-asia.com
ngs@
base-asia.com
 Tel:  +65 67757318  +60-3-8943 3252  +60-3-8943 3252
 

 

 

HMW DNA prep for Optical Mapping

                  Product Code                                   

Species    

Starting Amount/Type of Tissue 

Deliverable        

Turnaround Time

 

AEX-1001                   

Plants

10 grams of very young leaves, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

4 ultrapure agar plugs and PFG/CHEF run

2-3 weeks

AEX-1002

Insects

1-2 grams of larvae, starved for 36 hours, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

AEX-1003

Human cell lines

1-2 grams of cells, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

AEX-1004

Mammals

1-2 grams of liver tissue, starved for 36 hours, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

AEX-1005

Fish

0.5ml - 1ml of whole blood, collected in purple top vacuutainer tubes and stored at 4C, do not freeze (or collected in RNALater Solution as per Ambion Protocol).

AEX-1006                            

Marine organisms

Depending on organism and tissue type – Please contact us for assistance.

AEX-1007

Reptiles

Depending on organism and tissue type - Please contact us for assistance.

AEX-1008

Bacteria / yeast / fungi

Bacteria/Yeast 1-2g pellet frozen or agar stab/glycerol stock; Fungi protoplast 1-2g

 

 

 

 

HMW DNA prep for PacBio DNA sequencing

Product Code      

Species  

Starting Amount/Type of Tissue

Deliverable

Turnaround Time

 

AEX-2001                             

Plants

10 grams of very young leaves, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

20μg of DNA quantified by Qbit and PFG/CHEF run. Each isolation is sufficient for the PacBio run up to 2 SMRT cells.

1-2 weeks

AEX-2002

Insects

1-3 grams of larvae, starved for 36 hours, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

 

AEX-2003                                         

Human cell lines                   

1-3 grams of cells, starved for 36 hours, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

AEX-2004

Mammals

1-3 grams of liver tissue, starved for 36 hours, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

AEX-2005

Fish

1-3 ml of whole blood, collected in purple top vacuutainer tubes and stored at 4C, do not freeze (or collected in RNALater Solution as per Ambion Protocol).

AEX-2006

Marine organisms

Depending on organism and tissue type - Please contact us for assistance.

AEX-2007

Reptiles

Depending on organism and tissue type - Please contact us for assistance.

 

AEX-2008                                                                                      

Bacteria / yeast / fungi

Bacteria/Yeast 2-5g pellet frozen or agar stab/glycerol stock; Fungi 10g snap frozen

 

 

HMW DNA prep for BAC clone sequencing

Product Code                                         

Species

Starting Amount/Type of Tissue

Deliverable

Turnaround Time

 AEX-5001A

Plant Dicot/Eucot

20-40 grams of very young leaves, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

Engage in BAC Library with ~18-24 regular agar

plugs at ~5μg per plug and PFGE Photo

1-2 weeks

 

AEX-5001-B                       

Plant Monocot

40-50 grams of very young leaves, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

 

AEX-5002                

Insects

2- 3grams of larvae, starved for 36 hours, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

AEX-5003

Human cell lines

2-3 gram of cells (<500mg of cancer tumor cells), snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion

Protocol).

AEX-5004

Mammals

0.5-1 gram of liver tissue, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

AEX-5005

Fish

Fish 1-2 ml of whole blood, collected in purple top vacuutainer tubes and stored at 4C, do not freeze.

AEX-5006

Marine organisms

Depending on organism and tissue type – Please contact us for assistance.

 

AEX-5007                                                                       

Reptiles

Depending on organism and tissue type - Please contact us for assistance.

 

HMW DNA prep for GemCode Construction

Product Code              

Species

Starting Amount/Type of Tissue

Deliverable

Turnaround Time

AEX-6001                                                                          

Plants

2-3 grams of very young leaves, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

500ng to 1μg of DNA quantified by Qbit

1-2 weeks

AEX-6002

Insects

0.5-1 gram of larvae, starved for 36 hours, snap frozen in liquid N2, stored at -70C

& shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

AEX-6003                        

Human cell lines

0.5-1 gram of cells, snap frozen in liquid N2, stored at -70C & shipped on Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

AEX-6004

Mammals

0.5-1 gram of liver tissue, snap frozen in liquid N2, stored at -70C & shipped on

Dry Ice (or collected in RNALater Solution as per Ambion Protocol).

AEX-6005

Fish

0.5-1 ml of whole blood, collected in purple top vacuutainer tubes and stored at

4C, do not freeze.

AEX-6006

Marine organisms

Depending on organism and tissue type – Please contact us for assistance.

AEX-6007

Reptiles

Depending on organism and tissue type - Please contact us for assistance.

 

Library Construction

 

Product Code                

Service

Starting Amount/Type of Tissue

Deliverable

Turnaround Time

AEX-3001                                                  

PacBio 20 kb/30KB library preparation services (ready to run)

10 - 15 ug of DNA is needed as starting materials         

AEX provide DNA ready to load on RSII/Sequel

1-2 weeks

AEX-3003

GemCode Library Construction

100ng gDNA

One GemCode Library ready to load on any Illumina

2 weeks

 

BAC clones sequencing

 

AEX-4001                                       

BAC Clone sequencing

Client provides living BAC clone (agar stab or glycerol stock)

Fastq NGS data and assembly with minimum number of contigs

4-6 weeks

AEX-4002      

Client provides living BAC clone (96 well plate of glycerol stocks)

Please inquire.