Single Pass DNA Sequencing (SS1001, SS1002)

A full service DNA sequencing reaction encompasses the cycle sequencing reactions, dye terminator removal and subsequent analysis on our Genetic Analyzers. We also accept samples in 96-well plates. This is recommended for bulk orders, with a minimum of 80 reactions per plate.  

Customers need to send us their purified DNA (plasmid or PCR product) and specific primers individually. We strongly recommend 1.5mL of microtube to be used for Service Code SS1001; and PCR strip-capped 96-well plate for Service code: SS1002   

Sample Preparation Requirements for DNA Sequencing Reactions for local customers (Singapore and Malaysia)

Type of DNA Template

Recommended Concentration/ Reaction

Required Volume/ Reaction

Purified PCR Product [90-500bp]

5 ng/µL

10 uL

Purified PCR Product [501-1500bp]

20 ng/µL

Purified PCR Product [>1500bp]

30 ng/µL

Purified Plasmid (< 4kb)

50 ng/µL

Purified Plasmid (4kb to 10kb)

80 ng/µL

Purified Plasmid (>10kb)

100 ng/µL

Purified M13/ phagemid ssDNA

50 ng/µL

Purified Genomic DNA/ BAC

400 ng/µL

20 uL

 

Type of Primer

Required Concentration

Total of Reaction

Required Volume

Sequencing Primer

  • 18 to 25 bases
  • GC content: 40% to 60%
  • Tm = 55°C to 60°C
  • No significant hairpins (>3bp) and homodimerization
  • Desalted Primer (Brand: IDT) or Purified Primer (other Manufacturer)

10µM or 10pmol/µL or 50ng/uL

1

10uL

10

25uL

20

50uL

 

Sample Preparation Requirements for International Customers

We recommend that international customers send a larger volume of their samples to overcome loss of samples due to evaporation during the extended transportation to our facilities.

Type of DNA Template

Recommended Concentration/ Reaction

Required Volume/ Reaction

Purified PCR Product [90-500bp]

5 ng/µL

15 uL

Purified PCR Product [501-1500bp]

20 ng/µL

Purified PCR Product [>1500bp]

30 ng/µL

Purified Plasmid (< 4kb)

50 ng/µL

Purified Plasmid (4kb to 10kb)

80 ng/µL

Purified Plasmid (>10kb)

100 ng/µL

Purified M13/ phagemid ssDNA

50 ng/µL

Purified Genomic DNA/ BAC

400 ng/µL

25 uL

 

Type of Primer

Required Concentration

Total of Reaction

Required Volume

Sequencing Primer

  • 18 to 25 bases
  • GC content: 40% to 60%
  • Tm = 55°C to 60°C
  • No significant hairpins (>3bp) and homodimerization
  • Desalted Primer (Brand: IDT) or Purified Primer (other Manufacturer)

10µM or 10pmol/µL or 50ng/uL

1

10uL

10

25uL

20

50uL

To view list of complimentary Universal Primers provided by our DNA Sequencing facility, please click here.